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Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implications

  • Authors:
    • Wen G. Jiang
    • Andrew J. Sanders
    • Fiona Ruge
    • Keith G. Harding

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  • Published online on: December 1, 2011     https://doi.org/10.3892/etm.2011.402
  • Pages: 231-236
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Abstract

Interleukin (IL)-8 is a pro-inflammatory cytokine that has a direct effect on immune cells, including polymorphonuclear cells. Keratinocytes are a rich source of IL-8. However, there is little knowledge on the role of IL-8 in clinical wound healing and the direct biological effect of IL-8 on keratinocytes. In this study, the effect of recombinant human IL-8 (rhIL-8) on migration and adhesion was tested using HaCaT keratinocytes as a cell model. The cell functions were evaluated using impedance cell sensing. The expression of IL-8 receptor (IL-8R) transcripts in human skin and wounds (acute and chronic) was assessed using real-time transcript analysis. rhIL-8 significantly increased the migration of keratinocytes (3.5±0.3 for cells treated with IL-8 vs. 2.7±0.6 for controls; p=0.029). It is interesting to note that treatment of keratinocytes with IL-8 resulted in a marked shift in the responsive frequencies. IL-8 only resulted in a marginal increase in cell adhesion, which was particularly noticeable at high frequencies. The PLC-γ inhibitor completely eradicated the action of IL-8 on the migration of HaCaT cells. Using real time PCR, it was found that chronic wounds had significantly lower levels of the B form of the IL-8R (IL-8RB) (p=0.045) and marginally lower levels of the A form, IL-8RA, in comparison with acute wounds. Therefore, IL-8 has a direct and profound stimulatory effect on the migration of human keratinocytes, which is likely to occur via the PLC-γ pathway. Together with a reduced level of IL-8Rs in difficult-healing wounds, IL-8 has a clear prognostic and therapeutic value in wound healing.
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February 2012
Volume 3 Issue 2

Print ISSN: 1792-0981
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Spandidos Publications style
Jiang WG, Sanders AJ, Ruge F and Harding KG: Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implications. Exp Ther Med 3: 231-236, 2012.
APA
Jiang, W.G., Sanders, A.J., Ruge, F., & Harding, K.G. (2012). Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implications. Experimental and Therapeutic Medicine, 3, 231-236. https://doi.org/10.3892/etm.2011.402
MLA
Jiang, W. G., Sanders, A. J., Ruge, F., Harding, K. G."Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implications". Experimental and Therapeutic Medicine 3.2 (2012): 231-236.
Chicago
Jiang, W. G., Sanders, A. J., Ruge, F., Harding, K. G."Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implications". Experimental and Therapeutic Medicine 3, no. 2 (2012): 231-236. https://doi.org/10.3892/etm.2011.402