Bladder cancer-associated protein is suppressed in human cervical tumors
- Authors:
- Min Peng
- Tingbo Xie
- Juan Yu
- Bin Xu
- Qibin Song
- Xinxing Wu
View Affiliations
Affiliations: Oncology Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, P.R. China, Wuhan Institute of Biologic Products, Wuhan, Hubei, P.R. China, Institute of Virology, School of Medicine, Wuhan University, Wuhan, Hubei 430071, P.R. China
- Published online on: December 5, 2011 https://doi.org/10.3892/etm.2011.408
-
Pages:
336-340
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Abstract
Bladder cancer-associated protein (BLCAP) is downregulated in bladder cancer and has been identified as a prognostic biomarker for human bladder cancer. We previously reported that BLCAP mRNA is decreased in cervical cancer tissues, and overexpression of BLCAP was found to inhibit cell growth and induce apoptosis in the human cervical cancer HeLa cell line To investigate the BLCAP protein expression in cervical cancer and its potential clinical indications, we developed a polyclonal antibody against human BLCAP to assess the BLCAP protein expression in 30 cervical cancer tissues and 30 non-tumor cervical tissues from patients. Western blotting data showed that a single band of recombinant protein was probed by antiserum of BLCAP and no band was probed by pre-immune serum. BLCAP expression was significantly downregulated in cervical carcinoma tissues compared with its expression in the non-tumor cervical tissues. Moreover, cervical carcinoma tissues from patients with stage Ⅲ-Ⅳ had significantly lower BLCAP expression percentage compared with stage Ⅰ-Ⅱ. Similarly, a significantly lower BLCAP expression percentage was observed in moderately/poorly differentiated tumor tissues and in the tumor tissues from patients with lymphatic metastasis (LM) compared with well-differentiated tumor tissues and non-LM patients, respectively. Our results suggest that decreased BLCAP protein expression is associated with poor prognosis and it could be a potential bio-index to predict cervical tumor patient outcome.
View References
|
1.
|
K SzokeT SapyZ KrasznaiZ HernadiG SzladekG
VeressJ DillnerL GergelyJ KonyaModerate variation of the oncogenic
potential among high-risk human papillomavirus types in gynecologic
patients with cervical abnormalitiesJ Med
Virol71585592200310.1002/jmv.10526
|
|
2.
|
J BultenWJ MelchersMM Kooy-SmitsPC de
WildePJ PoddigheJC RobbenMV MacvilleLF MassugerJM BakkersAG
HanselaarNumerical aberrations of chromosome 1 in cervical
intraepithelial neoplasia are strongly associated with infection
with high-risk human papillomavirus typesJ
Pathol198300309200210.1002/path.1222
|
|
3.
|
A SarasinAn overview of the mechanisms of
mutagenesis and carcinogenesisMutat
Res54499106200310.1016/j.mrrev.2003.06.02414644312
|
|
4.
|
J YokotaTumor progression and
metastasisCarcinogenesis21497503200010.1093/carcin/21.3.49710688870
|
|
5.
|
JM MoreiraG OhlssonP GromovR SimonG
SauterJE CelisI GromovaBladder cancer-associated protein, a
potential prognostic biomarker in human bladder cancerMol Cell
Proteomics9161177201010.1074/mcp.M900294-MCP20019783793
|
|
6.
|
I GromovaP GromovJE CelisBc10: a novel
human bladder cancer-associated protein with a conserved genomic
structure downregulated in invasive cancerInt J
Cancer98539546200210.1002/ijc.1024411920613
|
|
7.
|
Z ZuoM ZhaoJ LiuG GaoX WuFunctional
analysis of bladder cancer-related protein gene: a putative
cervical cancer tumor suppressor gene in cervical carcinomaTumour
Biol27221226200610.1159/00009305716675915
|
|
8.
|
ZH ZuoM ZhaoJ LiuY WeiXX WuInhibitory
effect of bladder cancer related protein gene on hela cell
proliferationAi Zheng258118172006(In Chinese).
|
|
9.
|
PS HoltHD StoneRK GastCR GreeneApplication
of the agar gel precipitin test to detect antibodies to
Salmonella enterica serovar enteritidis in serum and egg
yolks from infected hensPoult
Sci7912461250200010.1093/ps/79.9.124611020067
|
|
10.
|
B StephenDRB EdgeAJCC Cancer Staging
Manual7th editionSpringer2009
|
|
11.
|
G De WildeN MertensE BooneB De VreeseJ Van
BeeumenW FiersG HaegemanExpression in Escherichia coli of
the death domain of the human p55 tumor necrosis factor
receptorProtein Expr Purif232262322001
|
|
12.
|
LI LobelS PollakJ KleinJW
LustbaderHigh-level bacterial expression of a natively folded,
soluble extracellular domain fusion protein of the human
luteinizing hormone/chorionic gonadotropin receptor in the
cytoplasm of escherichia
coliEndocrine14205212200110.1385/ENDO:14:2:205
|
|
13.
|
T WakagiT OshimaH ImamuraH
MatsuzawaCloning of the gene for inorganic pyrophosphatase from a
thermoacidophilic archaeon, sulfolobus sp Strain 7, and
overproduction of the enzyme by coexpression of tRNA for arginine
rare codonBiosci Biotechnol
Biochem6224082414199810.1271/bbb.62.24089972267
|
|
14.
|
RA SpanjaardK ChenJR WalkerJ van
DuinFrameshift suppression at tandem AGA and AGG codons by cloned
tRNA genes: assigning a codon to argU tRNA and T4 tRNA (Arg)Nucleic
Acids Res1850315036199010.1093/nar/18.17.50312205835
|
