Downregulation of keratins 8, 18 and 19 influences invasiveness of human cultured squamous cell carcinoma and adenocarcinoma cells

Uchiumi,A.; Yamashita,M.; Katagata,Y.

doi:10.3892/etm.2011.413

March 2012 Volume 3 Issue 3

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March 2012 Volume 3 Issue 3

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Article

Downregulation of keratins 8, 18 and 19 influences invasiveness of human cultured squamous cell carcinoma and adenocarcinoma cells

Authors:
- A. Uchiumi
- M. Yamashita
- Y. Katagata
View Affiliations / Copyright

Affiliations: Department of Molecular and Life Science, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori 036-8561, Japan
Pages: 443-448
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Published online on: December 9, 2011

https://doi.org/10.3892/etm.2011.413
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Abstract

Abstract. Keratin (K) expression index has been reported to be related to cell invasion activity in adenocarcinoma. In a previous study, we observed a negative correlation between K expression and cell invasion activity; i.e., when many Ks are expressed in the cells, the cell activity is low. To further elucidate the correlation between Ks and invasion activity, RNA interference experiments of K8, K18 and K19 were carried out to clarify the essential role of Ks using T24 and HEC-1 as typical squamous cell carcinoma and adenocarcinoma cells, respectively. K8 small interfering RNA (siRNA) was most effective against K18 and K19 expression and demonstrated the strongest effect on relative invasion activity among the siRNAs used. These results suggest that K8/K18 or K8/K19 filaments may play roles in internal cell structure and invasion activity. Moreover, K18 and K19 were capable of substituting for each other, and K18 or K19 formed filaments with K8. In addition, cells treated with K8 siRNA demonstrated high invasion activity, which was approximately double that observed with control siRNA in HEC-1 cells. The order of effects was K8>K19>K18 in the two cell lines. The above results suggest that K8 may play a signifiant role in invasive functions in epithelial and metastatic cells.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Downregulation of keratins 8, 18 and 19 influences invasiveness of human cultured squamous cell carcinoma and adenocarcinoma cells

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Abstract

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