Comparative proteomic analysis of the function and network mechanisms of MASPIN in human lung cells

Liu,Yao; Geng,Yi; Li,Kuanzhi; Wang,Fang; Zhou,Haiping; Wang,Wanhu; Hou,Jie; Liu,Wenchao

doi:10.3892/etm.2011.427

March 2012 Volume 3 Issue 3

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March 2012 Volume 3 Issue 3

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Article

Comparative proteomic analysis of the function and network mechanisms of MASPIN in human lung cells

Authors:
- Yao Liu
- Yi Geng
- Kuanzhi Li
- Fang Wang
- Haiping Zhou
- Wanhu Wang
- Jie Hou
- Wenchao Liu
View Affiliations / Copyright

Affiliations: Baoji Center Hospital of Shanxi Province, Baoji, Shanxi 721008, P.R. China, Department of Clinical Oncology, State Key Discipline of Cell Biology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, P.R. China
Pages: 470-474
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Published online on: December 22, 2011

https://doi.org/10.3892/etm.2011.427
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Abstract

MASPIN, which is also known as Serpin B5, is a novel tumor suppressor. Emerging evidence suggests that MASPIN acts as a multifaceted protein in various types of cancer, including prostate, breast and pancreatic cancer. It interacts with diverse groups of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis and angiogenesis, and is involved in mammary gland development. As MASPIN is a multifunctional factor in cancer pathways, its function remains poorly illuminated. In this study, we compared the protein profiles of LC5 cell lines with MASPIN overexpression and knockdown using comparative two-dimensional gel electrophoresis. The differences in protein expression, visualized as differences in spots, were identified by time‑of‑flight (TOF)/TOF mass spectometry. Significant differences were observed between overexpressing and knocked down cells, including eight spots that were unique and sixteen spots that were up- or down-regulated by more than 4-fold. Six genes, including Sdccag8, Ldoc1, SCAI, SDCCAG3, CT62 and NEDD9 were unique in MASPIN‑expressing cell lines, but absent in knock-out cell lines, in which most of them play a significant role in the invasion of cancer cells. Moreover, the Brms1 and CAGE1 genes were identified as being uniquely expressed in knocked down cell lines, which were associated with the development and progression of tumors. The data from this study shed some light on the function, as well as the general network mechanisms of MASPIN in lung cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparative proteomic analysis of the function and network mechanisms of MASPIN in human lung cells

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