Upregulation of TCTP expression in human skin squamous cell carcinoma increases tumor cell viability through anti-apoptotic action of the protein

Wu,Di; Guo,Ze; Min,Wei; Zhou,Bingrong; Li,Mingna; Li,Wei; Luo,Dan

doi:10.3892/etm.2011.439

Upregulation of TCTP expression in human skin squamous cell carcinoma increases tumor cell viability through anti-apoptotic action of the protein

Authors:
- Di Wu
- Ze Guo
- Wei Min
- Bingrong Zhou
- Mingna Li
- Wei Li
- Dan Luo
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Affiliations: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China, Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China
Published online on: December 28, 2011 https://doi.org/10.3892/etm.2011.439
Pages: 437-442

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Abstract

The translationally controlled tumor protein (TCTP) is an anti-apoptotic protein, which is highly expressed in several human cancer types. However, the role of TCTP in skin cancers, including squamous cell carcinoma (SCC), has not been investigated. In this study, we analyzed the expression of TCTP in cutaneous SCC samples using immunohistochemistry in two epidermoid SCC cell lines, A431 and SCL-1, using western blot analysis. We further investigated the role of TCTP in skin cancinogenesis by silencing the TPT1 gene using small interfering RNA (siRNA) in the SCC cell line A431. Our results demonstrated that TCTP was overexpressed in cutaneous SCC cells, compared to normal skin keratinocytes. In addition, the expression of TCTP in skin SCC significantly increased with the grade of malignancy. Western blot analysis further confirmed that the expression of TCTP in the cell lines, A431 and SCL-1, was significantly higher compared to that in the normal keratinocyte cell line, HaCaT. The expression of TCTP in A431 cells was significantly downregulated by transfection with our specifically designed TCTP siRNA. We found that downregulation of TCTP expression was associated with decreased cell proliferation and increased apoptosis in A431 cells. These results suggest that the TPT1 gene may be a potential therapeutic target in skin SCC through a siRNA approach.

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1.	H BohmR BenndorfM GaestelB GrossP NurnbergR KraftA OttoH BielkaThe growth-related protein P23 of the Ehrlich ascites tumor: translational control, cloning and primary structureBiochem Int1927728619892479380
2.	C BonnetE PerretX DumontA PicardD CaputG LenaersIdentification and transcription control of fission yeast genes repressed by an ammonium starvation growth arrestYeast162333200010.1002/(SICI)1097-0061(20000115)16:1%3C23::AID-YEA503%3E3.0.CO;2-A10620772
3.	SM MacDonaldT RafnarJ LangdonLM LichtensteinMolecular identification of an IgE-dependent histamine-releasing factorScience269688690199510.1126/science.75428037542803
4.	SH ChenPS WuCH ChouYT YanH LiuSY WengA knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific mannerMol Biol Cell1825252532200710.1091/mbc.E07-02-018817475776
5.	A TelermanR AmsonThe molecular programme of tumour reversion: the steps beyond malignant transformationNat Rev Cancer9206216200910.1038/nrc258919180095
6.	M TuynderL SusiniS PrieurS BesseG FiucciR AmsonA TelermanBiological models and genes of tumor reversion: cellular reprogramming through tpt1/TCTP and SIAH-1Proc Natl Acad Sci USA991497614981200210.1073/pnas.22247079912399545
7.	K OikawaT OhbayashiJ MimuraY Fujii-KuriyamaS TeshimaK RokutanK MukaiM KurodaDioxin stimulates synthesis and secretion of IgE-dependent histamine-releasing factorBiochem Biophys Res Commun290984987200210.1006/bbrc.2001.630211798171
8.	SR SturzenbaumP KilleAJ MorganIdentification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus1Biochim Biophys Acta1398294304199810.1016/S0167-4781(98)00077-39655922
9.	AS Vercoutter-EdouartX CzeszakM CrepinJ LemoineB BoillyX Le BourhisJP PeyratH HondermarckProteomic detection of changes in protein synthesis induced by fibroblast growth factor-2 in MCF-7 human breast cancer cellsExp Cell Res2625968200110.1006/excr.2000.5066
10.	JM LangdonBM VonakisSM MacDonaldIdentification of the interaction between the human recombinant histamine releasing factor/translationally controlled tumor protein and elongation factor-1 delta (also known as eElongation factor-1B beta)Biochim Biophys Acta1688232236200410.1016/j.bbadis.2003.12.007
11.	F LiD ZhangK FujiseCharacterization of fortilin, a novel antiapoptotic proteinJ Biol Chem2764754247549200110.1074/jbc.M10895420011598139
12.	N AmzallagBJ PasserD AllanicE SeguraC TheryB GoudR AmsonA TelermanTSAP6 facilitates the secretion of translationally controlled tumor protein/histamine-releasing factor via a nonclassical pathwayJ Biol Chem27946104461012200410.1074/jbc.M40485020015319436
13.	J JungM KimMJ KimJ KimJ MoonJS LimM KimK LeeTranslationally controlled tumor protein interacts with the third cytoplasmic domain of Na,K-ATPase alpha subunit and inhibits the pump activity in HeLa cellsJ Biol Chem2794986849875200410.1074/jbc.M40089520015383549
14.	Y YangF YangZ XiongY YanX WangM NishinoAn N-terminal region of translationally controlled tumor protein is required for its antiapoptotic activityOncogene2447784788200510.1038/sj.onc.120866615870695
15.	H LiuHW PengYS ChengHS YuanHF Yang-YenStabilization and enhancement of the antiapoptotic activity of mcl-1 by TCTPMol Cell Biol2531173126200510.1128/MCB.25.8.3117-3126.200515798198
16.	JC SanchezD SchallerF RavierO GolazS JaccoudM BeletTranslationally controlled tumor protein: a protein identified in several nontumoral cells including erythrocytesElectrophoresis18150155199710.1002/elps.1150180127
17.	L SusiniS BesseD DuflautA LespagnolC BeekmanG FiucciTCTP protects from apoptotic cell death by antagonizing bax functionCell Death Differ1512111220200810.1038/cdd.2008.1818274553
18.	TH HuCC HuangLF LiuPR LinSY LiuHW ChangExpression of hepatoma-derived growth factor in hepatocellular carcinomaCancer9814441456200310.1002/cncr.1165314508832
19.	SB RhoJH LeeMS ParkHJ ByunS KangSS SeoAnti-apoptotic protein TCTP controls the stability of the tumor suppressor p53FEBS Lett5852935201110.1016/j.febslet.2010.11.01421081126
20.	P GraidistM YazawaM TonganuntA NakatomiCC LinJY ChangFortilin binds Ca²⁺ and blocks Ca²⁺-dependent apoptosis in vivoBiochem J408181191200717705784