Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October 2012 Volume 4 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2012 Volume 4 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control

  • Authors:
    • Bo Zhang
    • Ying Shi
    • Tie‑Chi Lei
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 705-710
    |
    Published online on: August 16, 2012
       https://doi.org/10.3892/etm.2012.667
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:


Abstract

Active P‑glycoprotein (P‑gp) molecules have been shown to transport steroids out of peripheral lymphocytes, resulting in poor responses to systemic steroid therapy in patients with systemic lupus erythematosus (SLE). This study was carried out to investigate the correlation between the expression or activity of P‑gp in peripheral lymphocytes and disease control in SLE patients with a long history of systemic steroid treatment. A total of 60 SLE patients who had received systemic steroid treatment for longer than 6 months and 30 healthy subjects were monitored. SLE patients were subclassified into those with active and severely active forms of the disease according to their disease activity (estimated by SLEDAI-2000). The expression levels and activity of P‑gp in peripheral blood lymphocytes were determined. Lymphocytes, obtained from three patients with severely active SLE, with high levels of P‑gp expression were treated with cyclophosphamide, mycophenolic acid or emodin in vitro and Rh123‑efflux activity was measured. P‑gp expression in the peripheral lymphocytes of the SLE patients was significantly higher compared with that of the healthy controls, and a positive correlation between disease activity and P‑gp expression levels was observed in these 60 patients. A significant increase in P‑gp expression was observed in the severely active compared with the active SLE group. Treatment of lymphocytes with 100 µM cyclophosphamide or 100 µM emodin in vitro induced up to a 2‑fold increase in the mean fluorescence intensity, as detected by the Rh123‑efflux assay. In conclusion, the high expression levels of P‑gp in the peripheral lymphocytes of SLE patients leads to poor disease control by systemic steroids. Emodin, an active ingredient derived from Chinese herbs, possesses a promising effect for overcoming P‑gp-mediated steroid resistance by inhibiting the P-gp efflux function.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

D’Cruz DP, Khamashta MA and Hughes GR: Systemic lupus erythematosus. Lancet. 369:587–596. 2007.

2 

Parker BJ and Bruce IN: High dose methylprednisolone therapy for the treatment of severe systemic lupus erythematosus. Lupus. 16:387–393. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Wallace DJ: Advances in drug therapy for systemic lupus erythematosus. BMC Med. 29:772010. View Article : Google Scholar

4 

Barnes PJ: Mechanisms and resistance in glucocorticoid control of inflammation. J Steroid Biochem Mol Biol. 120:76–85. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Tsujimura S, Saito K, Nakayamada S, Nakano K and Tanaka Y: Clinical relevance of the expression of P-glycoprotein on peripheral blood lymphocytes to steroid resistance in patients with systemic lupus erythematosus. Arthritis Rheum. 52:1676–1683. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Richaud-Patin Y, Soto-Vega E, Jakez-Ocampo J and Llorente L: P-glycoprotein in autoimmune diseases. Autoimmun Rev. 3:188–192. 2004. View Article : Google Scholar

7 

Hochberg MC: Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus (letter). Arthritis Rheum. 40:17251997. View Article : Google Scholar : PubMed/NCBI

8 

Gladman DD, Ibañez D and Urowitz MB: Systemic lupus erythematosus disease activity index 2000. J Rheumatol. 29:288–291. 2002.PubMed/NCBI

9 

Yee CS, Farewell VT, Isenberg DA, et al: The use of Systemic Lupus Erythematosus Disease Activity Index-2000 to define active disease and minimal clinically meaningful change based on data from a large cohort of systemic lupus erythematosus patients. Rheumatology (Oxford). 50:982–988. 2011. View Article : Google Scholar

10 

Feng X, Wu H, Grossman JM, et al: Association of increased interferon-inducible gene expression with disease activity and lupus nephritis in patients with systemic lupus erythematosus. Arthritis Rheum. 54:2951–2962. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Petri M, Kim MY, Kalunian KC, et al: Combined oral contraceptives in women with systemic lupus erythematosus. N Engl J Med. 353:2550–2558. 2005. View Article : Google Scholar : PubMed/NCBI

12 

de la Fuente H, Baranda L, Hernández MI, et al: Lack of involvement of P-glycoprotein (P-gp) in pemphigus patients with poor response to steroid therapy. J Dermatol Sci. 28:219–226. 2002.PubMed/NCBI

13 

Grishanova AY, Melnikova EV, Kaledin VI, Nikolin VP and Lyakhovich VV: Possible role of P-glycoprotein in cyclophosphamide resistance of transplanted mouse RLS lymphosarcoma. Bull Exp Biol Med. 139:611–614. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Wang J, Figurski M, Shaw LM and Burckart GJ: The impact of P-glycoprotein and Mrp2 on mycophenolic acid levels in mice. Transpl Immunol. 19:192–196. 2008.(In English and Russian).

15 

Li J, Liu P, Mao H, Wanga A and Zhang X: Emodin sensitizes paclitaxel-resistant human ovarian cancer cells to paclitaxel-induced apoptosis in vitro. Oncol Rep. 21:1605–1610. 2009.PubMed/NCBI

16 

Guiducci C, Gong M, Xu Z, Gill M, et al: TLR recognition of self nucleic acids hampers glucocorticoid activity in lupus. Nature. 465:937–941. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Tsujimura S, Saito K, Tokunaga M, et al: Overcoming treatment unresponsiveness mediated by P-glycoprotein overexpression on lymphocytes in refractory active systemic lupus erythematosus. Mod Rheumatol. 15:28–32. 2005. View Article : Google Scholar

18 

Foote A, Briganti EM, Kipen Y, et al: Macrophage migration inhibitory factor in systemic lupus erythematosus. J Rheumatol. 31:268–273. 2004.PubMed/NCBI

19 

Hoi AY, Hickey MJ, Hall P, Yamana J, et al: Macrophage migration inhibitory factor deficiency attenuates macrophage recruitment, glomerulonephritis, and lethality in MRL/lpr mice. J Immunol. 177:5687–5696. 2006. View Article : Google Scholar

20 

Li X, Zhang FS, Zhang JH and Wang JY: Negative relationship between expression of glucocorticoid receptor alpha and disease activity: glucocorticoid treatment of patients with systemic lupus erythematosus. J Rheumatol. 37:316–321. 2010. View Article : Google Scholar

21 

Callaghan R, Crowley E, Potter S and Kerr ID: P-glycoprotein: so many ways to turn it on. J Clin Pharmacol. 48:365–378. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Wasilewska A, Zoch-Zwierz W, Pietruczuk M and Zalewski G: Expression of P-glycoprotein in lymphocytes from children with nephrotic syndrome, depending on their steroid response. Pediatr Nephrol. 21:1274–1280. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Barnes PJ and Adcock IM: Glucocorticoid resistance in inflammatory diseases. Lancet. 373:1905–1917. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Farrell RJ and Kelleher D: Glucocorticoid resistance in inflammatory bowel disease. J Endocrinol. 178:339–346. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Tsujimura S, Saito K, Nakayamada S and Tanaka Y: Etanercept overcomes P-glycoprotein-induced drug resistance in lymphocytes of patients with intractable rheumatoid arthritis. Mod Rheumatol. 20:139–146. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Maillefert JF, Maynadie M, Tebib JG, et al: Expression of the multidrug resistance glycoprotein 170 in the peripheral blood lymphocytes of rheumatoid arthritis patients. The percentage of lymphocytes expressing glycoprotein 170 is increased in patients treated with prednisolone. Br J Rheumatol. 35:430–435. 1996. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Shi Y and Lei TC: Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control. Exp Ther Med 4: 705-710, 2012.
APA
Zhang, B., Shi, Y., & Lei, T. (2012). Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control. Experimental and Therapeutic Medicine, 4, 705-710. https://doi.org/10.3892/etm.2012.667
MLA
Zhang, B., Shi, Y., Lei, T."Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control". Experimental and Therapeutic Medicine 4.4 (2012): 705-710.
Chicago
Zhang, B., Shi, Y., Lei, T."Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control". Experimental and Therapeutic Medicine 4, no. 4 (2012): 705-710. https://doi.org/10.3892/etm.2012.667
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Shi Y and Lei TC: Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control. Exp Ther Med 4: 705-710, 2012.
APA
Zhang, B., Shi, Y., & Lei, T. (2012). Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control. Experimental and Therapeutic Medicine, 4, 705-710. https://doi.org/10.3892/etm.2012.667
MLA
Zhang, B., Shi, Y., Lei, T."Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control". Experimental and Therapeutic Medicine 4.4 (2012): 705-710.
Chicago
Zhang, B., Shi, Y., Lei, T."Detection of active P‑glycoprotein in systemic lupus erythematosus patients with poor disease control". Experimental and Therapeutic Medicine 4, no. 4 (2012): 705-710. https://doi.org/10.3892/etm.2012.667
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team