Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome

Wei,Guo-Chao; Chen,Jing; Liu,Ai-Yun; Zhang,Miao; Liu,Xiao-Jun; Liu,Dan; Xu,Jun; Liu,Bing-Rong; Ling,Hong; Wu,Hua-Xing; Du,Ya-Ju

doi:10.3892/etm.2012.704

Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome

Authors:
- Guo-Chao Wei
- Jing Chen
- Ai-Yun Liu
- Miao Zhang
- Xiao-Jun Liu
- Dan Liu
- Jun Xu
- Bing-Rong Liu
- Hong Ling
- Hua-Xing Wu
- Ya-Ju Du
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Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Microbiology, Harbin Medical University, The Third Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Endoscopic Center, The Third Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
Published online on: September 11, 2012 https://doi.org/10.3892/etm.2012.704
Pages: 1039-1044

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Abstract

The aim of this study was to assess the genetic status of cagA, vacA subtype and iceA genotypes of Helicobacter pylori and the relationship with upper gastrointestinal diseases in Northeast China. Gastric biopsies were obtained from 378 patients with upper gastrointestinal diseases and 197 samples were used. The cagA, vacA alleles and iceA genotypes were determined by polymerase chain reaction. CagA was present in 176 (89.3%) of 197 patients. Of the 197 cases, 186 (94.4%) had vacA signal sequence s1c allele, 6 (3%) had s1a and 5 (2.5%) had s1b. The vacA s2 genotype was not detected in our study. VacA middle region sequences, m1 and m2, were found in 20 (10.2%) and 150 (76.1%), respectively. The allelic variant iceA1 (70.1%) was more prevalent than iceA2 (23.4%). The vacA allele s1am2 had a significant relationship with the presence of gastric cancer (p<0.05) and the iceA1 genotype was also associated with gastric cancer (p<0.05). These may be useful risk factors for upper gastrointestinal diseases.

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