Analysis of the expression levels of survivin and VEGF in patients with acute lymphoblastic leukemia

Yang,Man; Liu,Yongqiang; Lu,Shufang; Wang,Zhifen; Wang,Ran; Zi,Youmei; Li,Jingdong

doi:10.3892/etm.2012.769

Analysis of the expression levels of survivin and VEGF in patients with acute lymphoblastic leukemia

Authors:
- Man Yang
- Yongqiang Liu
- Shufang Lu
- Zhifen Wang
- Ran Wang
- Youmei Zi
- Jingdong Li
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Affiliations: Department of Hematology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Cardiovascular Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
Published online on: October 26, 2012 https://doi.org/10.3892/etm.2012.769
Pages: 305-307

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Abstract

The purpose of this study was to investigate the association between an inhibitor of apoptosis (survivin) and vascular endothelial growth factor (VEGF) expression in patients with acute lymphoblastic leukemia (ALL) prior to and following chemotherapy. Reverse transcription (RT)-PCR and western blotting were employed to analyze survivin and VEGF mRNA and protein expression. Moreover, the concentrations of survivin and VEGF were determined by enzyme-linked immunosorbent assay (ELISA). Our data revealed that survivin and VEGF were overexpressed in patients with ALL prior to treatment, while survivin levels were significantly decreased following treatment. In addition, there was a positive correlation between survivin and VEGF concentrations in plasma. In conclusion, analysis of survivin and VEGF expression levels may improve the clinical diagnosis and treatment of ALL.

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