Cell proliferation and invasion ability of human choriocarcinoma cells lessened due to inhibition of Sox2 expression by microRNA-145

Xu,Fuhui; Wang,Haipeng; Zhang,Xia; Liu,Te; Liu,Zhixue

doi:10.3892/etm.2012.781

Cell proliferation and invasion ability of human choriocarcinoma cells lessened due to inhibition of Sox2 expression by microRNA-145

Authors:
- Fuhui Xu
- Haipeng Wang
- Xia Zhang
- Te Liu
- Zhixue Liu
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Affiliations: School of Life Science and Technology, Tongji University, Shanghai 200092, P.R. China
Published online on: October 30, 2012 https://doi.org/10.3892/etm.2012.781
Pages: 77-84

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Abstract

To date, the mechanism underlying the development of human choriocarcinomas has not been elucidated. It is hypothesized that the Sox2 protein plays a pivotal role in the proliferation and invasion capacity of tumor cells. A microRNA (miR-145) was cloned and used to study the expression of Sox2 and its regulatory effect on the proliferation and invasion capacity of the human choriocarcinoma cell line JAR. In the present study, Sox2 mRNA and protein expression decreased in JAR and JEG-3 cells following transfection with the miR-145 expression virus. Cell proliferation assays indicated that miR-145 expression affected cell cycle regulation and suppressed the proliferation of choriocarcinoma cells in vitro. In addition, xenograft experiments confirmed the suppression of tumor growth in vivo due to cell cycle arrest. Therefore, endogenous mature miR-145 expression may have an important role in the pathogenesis of human choriocarcinomas via interference with the Sox2 target gene by epigenetic modification. This information is of potential significance for the identification of therapeutic targets in human choriocarcinoma.

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1	Bagley RG, Ren Y, Kurtzberg L, et al: Human choriocarcinomas: placental growth factor-dependent preclinical. Int J Oncol. 40:479–486. 2012.PubMed/NCBI
2	Monzo M, Navarro A, Bandres E, et al: Overlapping expression of microRNAs in human embryonic colon and colorectal cancer. Cell Res. 18:823–833. 2008. View Article : Google Scholar : PubMed/NCBI
3	Kim VN and Nam JW: Genomics of microRNA. Trends Genet. 22:165–173. 2006. View Article : Google Scholar : PubMed/NCBI
4	Hatfield SD, Shcherbata HR, Fischer KA, Nakahara K, Carthew RW and Ruohola-Baker H: Stem cell division is regulated by the microRNA pathway. Nature. 435:974–978. 2005. View Article : Google Scholar : PubMed/NCBI
5	Eder M and Scherr M: MicroRNA and lung cancer. N Engl J Med. 352:2446–2448. 2005. View Article : Google Scholar : PubMed/NCBI
6	Calin GA, Ferracin M, Cimmino A, et al: A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med. 353:1793–1801. 2005. View Article : Google Scholar : PubMed/NCBI
7	Houbaviy HB, Murray MF and Sharp PA: Embryonic stem cell-specific MicroRNAs. Dev Cell. 5:351–358. 2003. View Article : Google Scholar : PubMed/NCBI
8	Lanza G, Ferracin M, Gafà R, et al: mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer. Mol Cancer. 6:542007. View Article : Google Scholar : PubMed/NCBI
9	Chan JA, Krichevsky AM and Kosik KS: MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 65:6029–6033. 2005. View Article : Google Scholar : PubMed/NCBI
10	Schetter AJ, Leung SY, Sohn JJ, et al: MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA. 299:425–436. 2008. View Article : Google Scholar : PubMed/NCBI
11	Bandrés E, Cubedo E, Agirre X, et al: Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues. Mol Cancer. 5:292006.PubMed/NCBI
12	Volinia S, Calin GA, Liu CG, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI
13	Zhang L, Liu T, Huang Y and Liu J: microRNA-182 inhibits the proliferation and invasion of human lung adenocarcinoma cells through its effect on human cortical actin-associated protein. Int J Mol Med. 28:381–388. 2011.PubMed/NCBI
14	Chao A, Tsai CL, Wei PC, et al: Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma. Cancer Lett. 291:99–107. 2010. View Article : Google Scholar : PubMed/NCBI
15	Tay Y, Zhang J, Thomson AM, Lim B and Rigoutsos I: MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation. Nature. 455:1124–1128. 2008. View Article : Google Scholar : PubMed/NCBI
16	Chew JL, Loh YH, Zhang W, et al: Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Mol Cell Biol. 25:6031–6046. 2005. View Article : Google Scholar : PubMed/NCBI
17	Fong H, Hohenstein KA and Donovan PJ: Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cells. 26:1931–1938. 2008. View Article : Google Scholar : PubMed/NCBI
18	Xu N, Papagiannakopoulos T, Pan G, Thomson JA and Kosik KS: MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell. 137:647–658. 2009. View Article : Google Scholar : PubMed/NCBI
19	Li AS, Siu MK, Zhang H, et al: Hypermethylation of SOX2 gene in hydatidiform mole and choriocarcinoma. Reprod Sci. 15:735–744. 2008. View Article : Google Scholar : PubMed/NCBI
20	Nakatsugawa M, Takahashi A, Hirohashi Y, et al: SOX2 is over-expressed in stem-like cells of human lung adenocarcinoma and augments the tumorigenicity. Lab Invest. 91:1796–1804. 2011. View Article : Google Scholar : PubMed/NCBI
21	Liu T, Cheng W, Huang Y, Huang Q, Jiang L and Guo L: Human amniotic epithelial cell feeder layers maintain human iPS cell pluripotency via inhibited endogenous microRNA-145 and increased Sox2 expression. Exp Cell Res. 318:424–434. 2012. View Article : Google Scholar : PubMed/NCBI
22	Eis PS, Tam W, Sun L, et al: Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc Natl Acad Sci USA. 102:3627–3632. 2005. View Article : Google Scholar : PubMed/NCBI
23	Zhang Y, Chao T, Li R, et al: MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a. J Mol Med (Berl). 87:43–51. 2009. View Article : Google Scholar : PubMed/NCBI
24	Cheng W, Liu T, Jiang F, et al: microRNA-155 regulates angiotensin II type 1 receptor expression in umbilical vein endothelial cells from severely pre-eclamptic pregnant women. Int J Mol Med. 27:393–399. 2011.PubMed/NCBI
25	Gupta RA, Shah N, Wang KC, et al: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 464:1071–1076. 2010. View Article : Google Scholar : PubMed/NCBI
26	Jiang F, Liu T, He Y, et al: MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo. BMC Cancer. 11:4252011. View Article : Google Scholar : PubMed/NCBI
27	Carro MS, Lim WK, Alvarez MJ, et al: The transcriptional network for mesenchymal transformation of brain tumours. Nature. 463:318–325. 2010. View Article : Google Scholar : PubMed/NCBI
28	Kluiver J, Haralambieva E, de Jong D, et al: Lack of BIC and microRNA miR-155 expression in primary cases of Burkitt lymphoma. Genes Chromosomes Cancer. 45:147–153. 2006. View Article : Google Scholar : PubMed/NCBI
29	Martin MM, Lee EJ, Buckenberger JA, Schmittgen TD and Elton TS: MicroRNA-155 regulates human angiotensin II type 1 receptor expression in fibroblasts. J Biol Chem. 281:18277–18284. 2006. View Article : Google Scholar : PubMed/NCBI