Involvement of mast cell chymase in burn wound healing in hamsters

Dong,Xianglin; Geng,Zhongli; Zhao,Yang; Chen,Junjie; Cen,Ying

doi:10.3892/etm.2012.836

Involvement of mast cell chymase in burn wound healing in hamsters

Authors:
- Xianglin Dong
- Zhongli Geng
- Yang Zhao
- Junjie Chen
- Ying Cen
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Affiliations: Department of Burns and Plastic Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uigur Autonomous Region 830054, P.R. China, Department of Breast, Head and Neck Surgery, Affiliated Tumour Hospital, Xinjiang Medical University, Urumqi, Xinjiang Uigur Autonomous Region 830011, P.R. China, Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: November 27, 2012 https://doi.org/10.3892/etm.2012.836
Pages: 643-647

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Abstract

Mast cells play a significant role in the late stage of wound healing following burn injuries. In the present study, the possible role of mast cell chymase in burn wound healing was examined using a mast cell membrane stabilizer, ketotifen, in hamsters. A total of 28 hamsters were randomly divided into two groups (n=14), termed as the control and ketotifen groups. A deep partial‑thickness burn injury was made on the back skin of the hamsters. The control group was orally administered physiological saline (1 ml) and the ketotifen group was orally administered ketotifen (4 mg/kg) once daily, two days prior to and two days subsequent to the burn. The results showed that concentrations of angiotensin II (Ang II), TGF‑β1, collagens I and III and interleukin (IL)‑1β were significantly decreased in the ketotifen group compared with those in the control group. However, there was no significant difference in fibroblast apoptosis between the two groups. The release of mast cell chymase was inhibited by the mast cell membrane stabilizer ketotifen. Taken together, these results suggest that mast cell chymase may participate in the process of burn wound healing. Chymase may therefore be a promising therapeutic target for the treatment of burn wounds.

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