Shuangtengbitong tincture treatment of collagen‑induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB

Chu,Kedan; Zheng,Haiyin; Li,Huang; Zhang,Yuqin; Zhang,Xun; Xu,Wei; Chen,Lidian

doi:10.3892/etm.2012.853

Shuangtengbitong tincture treatment of collagen‑induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB

Authors:
- Kedan Chu
- Haiyin Zheng
- Huang Li
- Yuqin Zhang
- Xun Zhang
- Wei Xu
- Lidian Chen
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Affiliations: Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Integrative Medicine College, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Faculty of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China
Published online on: December 5, 2012 https://doi.org/10.3892/etm.2012.853
Pages: 423-428

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Abstract

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease and may lead to joint damage, synovial membrane destruction and cartilage and bone damage. RA is closely associated with increased expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α (TNF-α) and nuclear transcription factor-κB (NF-κB). Therefore, inhibition of the expression of IL-6, IL-8, TNF-α and NF-κB is a promising strategy for the development of novel anti-RA therapies. The aim of this study was to investigate the effect of shuangtengbitong tincture (STBT) on the expression of IL-6, IL-8, TNF-α and NF-κB in synovial tissues of rats with collagen-induced arthritis (CIA). STBT as a clinical prescription created at Fujian University of Traditional Chinese Medicines (TCM) Affiliated People's Hospital has been shown to be clinically effective in the treatment of RA. The model of Wistar rats with CIA was created using bovine type II collagen. The two treatment groups with CIA were administered STBT (1 ml per time) or Votalin (~1 cm per time) for ~1 month continuously. Following treatment, STBT suppressed paw swelling signiﬁcantly (P<0.05) compared with the model group. STBT also improved pathological changes, STBT-treated rats showed a signiﬁcant improvement in synovial hyperplasia, inﬂammatory inﬁltration, cartilage and bone destruction and other symptoms. The protein expression levels of IL-6, IL-8, TNF-α and NF-κB were markedly suppressed in synovial tissues of STBT-treated and Votalin-treated rats. Our findings demonstrate for the first time that STBT markedly reduces paw swelling, improves pathological changes and increases the expression of IL-6, IL-8, TNF-α and NF-κB in synovial tissues of CIA rats, which may partially explain the anti-RA activity of STBT.

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