Taurine detected using high‑resolution magic angle spinning 1H nuclear magnetic resonance: A potential indicator of early myocardial infarction

Yang,Yunlong; Yang,Lin; Zhang,Yue; Gu,Xinghua; Xu,Danling; Fang,Fang; Sun,Aijun; Wang,Keqiang; Yu,Yihua; Zuo,Ji; Ge,Junbo

doi:10.3892/etm.2012.857

Taurine detected using high‑resolution magic angle spinning 1H nuclear magnetic resonance: A potential indicator of early myocardial infarction

Authors:
- Yunlong Yang
- Lin Yang
- Yue Zhang
- Xinghua Gu
- Danling Xu
- Fang Fang
- Aijun Sun
- Keqiang Wang
- Yihua Yu
- Ji Zuo
- Junbo Ge
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Affiliations: Zhongshan Hospital of Fudan University, Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China, Research Center, Eye __AMB__ ENT Hospital of Fudan University, Shanghai 200031, P.R. China, Shanghai Key Laboratory of Functional Magnetic Resonance Imaging, Physics Department, East China Normal University, Shanghai 200062, P.R. China, Department of Cellular __AMB__ Genetic Medicine, Shanghai Medical School, Fudan University, Shanghai 200032, P.R. China
Published online on: December 13, 2012 https://doi.org/10.3892/etm.2012.857
Pages: 683-682

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Abstract

Magnetic resonance spectroscopy (MRS) is a unique non‑invasive method for detecting cardiac metabolic changes. However, MRS in cardiac diagnosis is limited due to insensitivity and low efficiency. Taurine (Tau) is the most abundant free amino acid in the myocardium. We hypothesized that Tau levels may indicate myocardial ischemia and early infarction. Sprague‑Dawley rats were divided into seven groups according to different time points during the course of myocardial ischemia, which was induced by left anterior descending coronary artery ligation. Infarcted myocardial tissue was obtained for high‑resolution magic angle spinning 1H nuclear magnetic resonance (NMR) analysis. Results were validated via high‑performance liquid chromatography. The Tau levels in the ischemic myocardial tissue were reduced significantly within 5 min compared with those in the control group (relative ratio from 20.27±6.48 to 8.81±0.04, P<0.05) and were maintained for 6 h post‑ischemia. Tau levels declined more markedly (56.5%) than creatine levels (48.5%) at 5 min after ligation. This suggests that Tau may have potential as an indicator in the early detection of myocardial ischemia by 1H MRS.

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