Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration

Jin,Kai; Ren,Dong-Dong; Chi,Fang-Lu; Yang,Juan-Mei; Huang,Yi-Bo; Li,Wen

doi:10.3892/etm.2013.1089

Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration

Authors:
- Kai Jin
- Dong-Dong Ren
- Fang-Lu Chi
- Juan-Mei Yang
- Yi-Bo Huang
- Wen Li
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Affiliations: Department of Otolaryngology, Otology and Skull Base Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China, Department of Lab Centre, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China
Published online on: April 29, 2013 https://doi.org/10.3892/etm.2013.1089
Pages: 177-183

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Abstract

The aim of this study was to investigate the effects of actin depolymerizing factor (ADF)/destrin and position changes of kinetosomes in the development of hair cells following Atoh1-induced ectopic regeneration in the basilar membrane of mice. We observed through immunofluorescence at various time-points the expression of ADF/destrin and the specific kinetosome marker, γ-tubulin, in hair cells following ectopic regeneration induced by adenovirus transfection, overexpression of Atoh1 and in vitro culture. Changes of ADF/destrin distribution and kinetosome position during in vitro culture of new hair cells [Myo7a(+)] following Atoh1-induced ectopic regeneration are consistent with the changes in ADF/destrin expression and the polar migration of kinetosomes in hair cells of the cochlear sensory epithelium in normal development. ADF/destrin is involved in the development of the auditory epithelium and the development and structural rearrangement of ectopically regenerated hair cells in mammals. The kinetosomes of hair cells following Atoh1‑induced ectopic regeneration show positional changes in vitro at different time-points.

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