Induction of apoptosis by sarijang, a bamboo salt sauce, in U937 human leukemia cells through the activation of caspases

  • Authors:
    • Eun‑A Choi
    • Cheol Park
    • Min‑Ho Han
    • Jun Hyuk Lee
    • Gi‑Young  Kim
    • Byung Tae Choi
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: June 10, 2013     https://doi.org/10.3892/etm.2013.1151
  • Pages: 381-387
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Abstract

Sarijang is a bamboo salt soy sauce, containing extracts of Rhynchosia nulubilis, sulfur‑fed duck, dried bark of Ulmus davidiana and Allium sativum, which has been demonstrated to exert anti‑inflammatory and antitumor activity. However, the cellular and molecular mechanisms of action of sarijang have not yet been elucidated. In the present study, we investigated the pro‑apoptotic effects of sarijang in an in vitro U937 human leukemia cell model. Treatment with sarijang resulted in a concentration‑dependent growth inhibition of the cells, coupled with the characteristic morphological features of apoptosis. The induction of the apoptotic cell death of the U937 cells by sarijang exhibited a correlation with the upregulation of death receptor 4 (DR4), the downregulation of members of the inhibitor of apoptosis protein (IAP) family, including survivin and cellular IAP (cIAP)‑1, and the cleavage of Bid. Apoptosis‑inducing concentrations of sarijang also induced the activation of caspases (caspase‑3, ‑8 and ‑9), accompanied by proteolytic degradation of poly(ADP‑ribose)‑polymerase, β‑catenin and phospholipase C‑γ1. However, the apoptosis induced by sarijang was significantly inhibited by z‑VED‑fmk, a pan‑caspase inhibitor, which demonstrated the importance of caspases in the process. These results suggested that sarijang may be a potential chemotherapeutic agent for use in the control of U937 human leukemia cells. Further studies are required to identify the active compounds in sarijang.
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August 2013
Volume 6 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Choi EA, Park C, Han MH, Lee J, Kim GY, Choi BT and Choi YH: Induction of apoptosis by sarijang, a bamboo salt sauce, in U937 human leukemia cells through the activation of caspases. Exp Ther Med 6: 381-387, 2013.
APA
Choi, E., Park, C., Han, M., Lee, J., Kim, G., Choi, B.T., & Choi, Y.H. (2013). Induction of apoptosis by sarijang, a bamboo salt sauce, in U937 human leukemia cells through the activation of caspases. Experimental and Therapeutic Medicine, 6, 381-387. https://doi.org/10.3892/etm.2013.1151
MLA
Choi, E., Park, C., Han, M., Lee, J., Kim, G., Choi, B. T., Choi, Y. H."Induction of apoptosis by sarijang, a bamboo salt sauce, in U937 human leukemia cells through the activation of caspases". Experimental and Therapeutic Medicine 6.2 (2013): 381-387.
Chicago
Choi, E., Park, C., Han, M., Lee, J., Kim, G., Choi, B. T., Choi, Y. H."Induction of apoptosis by sarijang, a bamboo salt sauce, in U937 human leukemia cells through the activation of caspases". Experimental and Therapeutic Medicine 6, no. 2 (2013): 381-387. https://doi.org/10.3892/etm.2013.1151