Assessment of IL‑6, IL‑8 and TNF‑α levels in the gingival tissue of patients with periodontitis

Noh,Min  Ki; Jung,Min; Kim,Soo  Hwan; Lee,Seo  Ra; Park,Ki  Ho; Kim,Dong  Hwan; Kim,Hanna  Hyun; Park,Young  Guk

doi:10.3892/etm.2013.1222

Assessment of IL‑6, IL‑8 and TNF‑α levels in the gingival tissue of patients with periodontitis

Authors:
- Min Ki Noh
- Min Jung
- Soo Hwan Kim
- Seo Ra Lee
- Ki Ho Park
- Dong Hwan Kim
- Hanna Hyun Kim
- Young Guk Park
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Affiliations: Department of Orthodontics, School of Dentistry, Kyung Hee University, Seoul 130‑701, Republic of Korea, College of Arts and Science, New York University, New York, NY 10012, USA
Published online on: July 15, 2013 https://doi.org/10.3892/etm.2013.1222
Pages: 847-851

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Abstract

In periodontal diseases, inflammatory mediators, including interleukin (IL)‑6, IL‑8 and tumor necrosis factor‑α (TNF‑α), may promote the degeneration of inflamed periodontal tissues. In previous studies, levels of these three cytokines were demonstrated to be elevated in inflammatory gingival tissues and gingival crevicular fluid. The aim of the present study was to quantify IL‑6, IL‑8 and TNF‑α levels in the human gingival tissues of patients with periodontitis and to assess the correlation of these three cytokines with each other. In this study, human gingival tissues from 19 patients with periodontitis (male, n=14; female, n=5) were collected. The tissues were homogenized, centrifuged and the protein in the supernatant was quantified. Enzyme‑linked immunosorbent assay (ELISA) was used in the measurement of the IL‑6, IL‑8 and TNF‑α levels, and the mean levels were observed to be 8.41±0.25, 34.01±1.09 and 20.70±0.31 pg/ml, respectively. The mean levels of IL‑8 were higher than those of the other two cytokines. In each sample, the level of TNF‑α expression was consistently high, with little difference between the results, which contrasted with the fluctuations in IL‑6 and IL‑8 levels. The expression of the two ILs (IL‑6 and IL‑8) showed a positive correlation (r=0.932, P=0.01), whereas TNF‑α levels were not correlated with IL‑6 or IL‑8 levels. These results suggest that IL‑6, IL‑8 and TNF‑α may be relevant in the pathophysiology of periodontitis, and the measurement of these cytokines may be beneficial in the identification of patients with periodontitis.

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