Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP‑2 and MMP‑9 in rats with endometriosis
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- Published online on: September 26, 2013 https://doi.org/10.3892/etm.2013.1316
- Pages: 1385-1389
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Abstract
The aim of this study was to explore the effect of a traditional Chinese medicine (Xiaochaihu Tang, XCHT) on the expression of matrix metalloproteinase‑2 (MMP‑2) and MMP‑9 in rats with endometriosis (EMs). A total of 48 specific‑pathogen‑free (SPF) female Sprague‑Dawley (SD) rats were randomly divided into control (n=8) and EMs (n=40) groups. The EMs model was established using a surgical procedure. At 21 days, the rats with EMs were screened and divided into four subgroups (n=8): the model control, low‑dose (7.5 g/kg) XCHT‑treated, high‑dose (15 g/kg) XCHT‑treated and gestrinone‑treated (0.5 mg/kg) groups. Following 21 days of treatment, the rats were sacrificed. Reverse transcription‑polymerase chain reaction (RT‑PCR) and western blotting were used to examine the mRNA and protein levels of MMP‑2 and MMP‑9 in the endometrium. The expression levels of MMP‑2 and MMP‑9 were significantly increased in the rats with EMs compared with those in normal rats. Moreover, XCHT was able to significantly inhibit the expression of MMP‑2 and MMP‑9 compared with that in the model control group. In conclusion, XCHT was able to decrease the expression of MMP‑2 and MMP‑9 in the ectopic endometrium. The present results may provide a potential theoretical basis for the therapy of EMs.
