Forsythiaside attenuates lipopolysaccharide‑induced inflammatory responses in the bursa of Fabricius of chickens by downregulating the NF‑κB signaling pathway

Cheng,Guangdong; Zhao,Yulian; Li,He; Wu,Yue; Li,Xianxian; Han,Qiang; Dai,Chongshan; Li,Yanhua

doi:10.3892/etm.2013.1378

Forsythiaside attenuates lipopolysaccharide‑induced inflammatory responses in the bursa of Fabricius of chickens by downregulating the NF‑κB signaling pathway

Authors:
- Guangdong Cheng
- Yulian Zhao
- He Li
- Yue Wu
- Xianxian Li
- Qiang Han
- Chongshan Dai
- Yanhua Li
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Affiliations: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China
Published online on: October 31, 2013 https://doi.org/10.3892/etm.2013.1378
Pages: 179-184

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Abstract

Forsythiaside, a phenylethanoside product isolated from air‑dried fruits of Forsythia suspensa, has been demonstrated to exhibit antioxidant, antibacterial and anti‑inflammatory activities in vitro. However, its mechanism and the effects of lipopolysaccharide (LPS)‑induced injury on the bursa of Fabricius (BF) of chickens are poorly understood. The present study aimed to investigate the anti‑inflammatory effects of forsythiaside on LPS‑induced acute inflammation. In addition, the potential molecular mechanisms of forsythiaside were analyzed in the BF, a special immune organ in chickens. Forty 15‑day‑old chickens were randomly divided into control, LPS and LPS plus forsythiaside (30 or 60 mg/kg) groups (n=10 for each group). In the LPS plus forsythiaside (30 or 60 mg/kg) groups, the chickens were orally administered with forsythiaside at doses of 30 and 60 mg/kg for seven days. At 21 days old, the chickens were intravenously injected with 200 µg/kg body weight LPS. Chickens in the control and LPS groups were only administered with vehicle or LPS, respectively, at day 21. At 3 h post‑injection, the body temperature and nitric oxide (NO) levels were analyzed. In addition, the levels and mRNA expression of pro‑inflammatory cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6) and IL‑1β, and the mRNA expression of nuclear factor‑κB (NF‑κB), cyclooxygenase‑2 (COX‑2) and inducible NO synthase (iNOS), were examined in the BFs isolated from the chickens. The results revealed that forsythiaside was able to attenuate the LPS‑induced inflammatory responses in the BFs of the chickens. The mechanisms by which forsythiaside exerted its anti‑inflammatory effect were found to correlate with the inhibition of IL‑6, IL‑1β, TNF‑α and COX‑2 production, via the inactivation of NF‑κB, indicating that the NF‑κB‑iNOS‑NO signaling pathway may be important in this process.

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