A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats

Kwak,Kyung-Hwa; Jung,Hoon; Park,Jun Mo; Yeo,Jin-Seok; Kim,Hyunjee; Lee,Hyung Chul; Byun,Sung Hye; Kim,Jong-Chan; Park,Sung-Sik; Lim,Dong Gun

doi:10.3892/etm.2013.1440

A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats

Authors:
- Kyung-Hwa Kwak
- Hoon Jung
- Jun Mo Park
- Jin-Seok Yeo
- Hyunjee Kim
- Hyung Chul Lee
- Sung Hye Byun
- Jong-Chan Kim
- Sung-Sik Park
- Dong Gun Lim
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Affiliations: Department of Anesthesiology and Pain Medicine, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea, Department of Anesthesiology and Pain Medicine, Keimyung University Dongsan Medical Center, Daegu 700‑721, Republic of Korea
Published online on: December 9, 2013 https://doi.org/10.3892/etm.2013.1440
Pages: 508-512

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Abstract

The contributions of superoxide and nitric oxide to ischemia/reperfusion (I/R)‑induced neuropathic pain have previously been demonstrated in an animal model that mimics the symptoms of complex regional pain syndrome type I (CRPS I). Targeting peroxynitrite, which is the product of their interaction, may provide effective treatments for I/R‑induced neuropathic pain. In this study, the effect of the peroxynitrite decomposition catalyst FeTMPyP [5,10,15,20‑tetrakis (N‑methyl‑4'‑pyridyl)porphyrinato iron (III)], administered at doses of 1, 3 and 10 mg/kg via intraperitoneal injection 30 min prior to reperfusion, was evaluated in rats with chronic post‑ischemic pain. The pain behavior of the rats was tested with a von Frey filament. Phosphorylation of N‑methyl‑D‑aspartate (NMDA) receptors in the L4/6 section of the spinal cord was measured on the third day following reperfusion by western blotting. The rats treated with 3 or 10 mg/kg FeTMPyP demonstrated significant increases in their paw withdrawal thresholds and decreased levels of phosphorylated NMDA receptor subunit 1 compared with those of the vehicle group (all P<0.001). These findings suggest that nitrosative stress, specifically that associated with peroxynitrite, may be involved in the mechanical allodynia and central sensitization that are associated with CRPS I and may provide a rationale for CRPS I treatment strategies using peroxynitrite decomposition catalysts.

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