Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells

Song,Jia‑Le; Zhao,Xin; Wang,Qiang

doi:10.3892/etm.2013.885

Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells

Authors:
- Jia‑Le Song
- Xin Zhao
- Qiang Wang
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Affiliations: Department of Food Science and Nutrition, Pusan National University, Busan 609‑735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
Published online on: January 7, 2013 https://doi.org/10.3892/etm.2013.885
Pages: 947-951

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Abstract

The present study was designed to investigate the protective effect of hot water extracts from Quercus salicina leaves (QSWE) on alloxan‑induced oxidative stress in HIT‑T15 Syrian hamster pancreatic insulinoma cells. The HIT‑T15 cells were treated with alloxan (1 mM) for 1 h and then co‑incubated with the QSWE for 24 h. Alloxan significantly decreased the viability of the HIT-T15 cells (P<0.05). QSWE did not exhibit significantly cytotoxic effects and increased the viability of the HIT‑T15 cells in a concentration‑dependent manner. To further investigate the protective effects of QSWE on alloxan‑induced oxidative stress in HIT‑T15 cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH‑px), were analyzed. QSWE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that QSWE exerted cytoprotective activity against alloxan‑induced oxidative stress in HIT‑T15 cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzyme activity. In addition, QSWE also increased the insulin secretion activity of the alloxan‑treated HIT‑T15 cells.

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