Studies on the effect of adenosine on calcium oscillation in hippocampal neurons

Chen,Jinbo; Wang,Yuan; Wang,Yuliang; Yi,Xiangming; Ge,Ruli

doi:10.3892/etm.2013.973

Studies on the effect of adenosine on calcium oscillation in hippocampal neurons

Authors:
- Jinbo Chen
- Yuan Wang
- Yuliang Wang
- Xiangming Yi
- Ruli Ge
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Affiliations: Department of Neurology, The Affiliated Hospital of Binzhou Medical University, Binzhou 256603, P.R. China
Published online on: February 25, 2013 https://doi.org/10.3892/etm.2013.973
Pages: 1165-1168

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Abstract

Adenosine (Ade) is an antiepileptic agent. In order to investigate the possible mechanism of action of Ade, its effect on calcium (Ca2+) oscillations in hippocampal neurons of Sprague Dawley (SD) rats was explored. Primary hippocampal neurons were cultured from suckling neonatal SD rats. Cells were cultured for 7-9 days and the Ca2+ oscillations in response to perfusion with Ade were detected using confocal laser scanning microscopy in combination with Fluo-3/AM labeling. This study found that Ade inhibits the spontaneous synchronized Ca2+ oscillation frequency and amplitude in mature hippocampal neurons and such inhibition depends on the Ade dosage level to a certain extent. Ade also had a significant inhibitory effect on high potassium-induced Ca2+ oscillation frequency and amplitude. Ade had a significant inhibitory effect on high-voltage-activated Ca2+ channel-mediated Ca2+ influx and Ca2+ oscillations in neurons. This may be one of the mechanisms for Ade to exert antiepileptic effects as an endogenous substance.

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