Effects of the antihypertensive drug benidipine on osteoblast function in vitro

  • Authors:
    • Baixiang Wang
    • Ming Bi
    • Zhen Zhu
    • Lei Wu
    • Jingyun Wang
  • View Affiliations

  • Published online on: January 3, 2014     https://doi.org/10.3892/etm.2014.1475
  • Pages: 649-653
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Abstract

The dihydropyridine‑type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro. Experiments were designed and performed, including an MTT assay, reverse transcription‑polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1x10‑6 to 1x10‑9 M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage.
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2014-March
Volume 7 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wang B, Bi M, Zhu Z, Wu L and Wang J: Effects of the antihypertensive drug benidipine on osteoblast function in vitro. Exp Ther Med 7: 649-653, 2014.
APA
Wang, B., Bi, M., Zhu, Z., Wu, L., & Wang, J. (2014). Effects of the antihypertensive drug benidipine on osteoblast function in vitro. Experimental and Therapeutic Medicine, 7, 649-653. https://doi.org/10.3892/etm.2014.1475
MLA
Wang, B., Bi, M., Zhu, Z., Wu, L., Wang, J."Effects of the antihypertensive drug benidipine on osteoblast function in vitro". Experimental and Therapeutic Medicine 7.3 (2014): 649-653.
Chicago
Wang, B., Bi, M., Zhu, Z., Wu, L., Wang, J."Effects of the antihypertensive drug benidipine on osteoblast function in vitro". Experimental and Therapeutic Medicine 7, no. 3 (2014): 649-653. https://doi.org/10.3892/etm.2014.1475