Effects of the antihypertensive drug benidipine on osteoblast function in vitro

Wang,Baixiang; Bi,Ming; Zhu,Zhen; Wu,Lei; Wang,Jingyun

doi:10.3892/etm.2014.1475

Effects of the antihypertensive drug benidipine on osteoblast function in vitro

Authors:
- Baixiang Wang
- Ming Bi
- Zhen Zhu
- Lei Wu
- Jingyun Wang
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Affiliations: Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China, Department of and Comprehensive Treatment, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Prosthodontics, Zhenjiang Stomatological Hospital, Zhenjiang, Jiangsu 212002, P.R. China, Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: January 3, 2014 https://doi.org/10.3892/etm.2014.1475
Pages: 649-653

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Abstract

The dihydropyridine‑type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro. Experiments were designed and performed, including an MTT assay, reverse transcription‑polymerase chain reaction, western blot analysis, alkaline phosphatase activity measurements and alizarin red S staining. The results demonstrated that BD promoted osteoblast proliferation and osteogenic differentiation at concentrations from 1x10‑6 to 1x10‑9 M by upregulating Runx2, BMP2 and OCN gene expression levels. Overall, BD at appropriate concentrations has been demonstrated to have positive effects on osteoblast function in addition to its conventional clinical usage.

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