Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion

  • Authors:
    • Ping Zhang
    • Zhen Huang
    • Hai-Qing Yan
    • Lin-Lin Su
    • Yong-Kun Gui
    • Hai-Xia Lv
    • Bin Zhu
    • Tong Li
  • View Affiliations

  • Published online on: January 10, 2014     https://doi.org/10.3892/etm.2014.1483
  • Pages: 657-662
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Abstract

The aim of the present study was to provide a simple method of establishing a rat model for focal cerebral ischemia‑reperfusion (FCIR). The suture‑occluded method was used to establish FCIR in male Sprague‑Dawley rats. An incision was made over the bifurcation of the common carotid artery (CCA), through which a suture was inserted up to the internal carotid artery (ICA). The suture remained in the skin subsequent to model establishment and was withdrawn to the CCA to enable reperfusion. The reliability of the rat model was assessed via analysis of nerve function, tetrazolium (TTC) staining and pathological examination. Following FCIR in rats, the resulting neurological impairments were observed. TTC staining revealed infarcts and pathological examination revealed typical pathological changes. This modified method was simple, reliable and, therefore, may be used to investigate FCIR.
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2014-March
Volume 7 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang P, Huang Z, Yan H, Su L, Gui Y, Lv H, Zhu B and Li T: Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion. Exp Ther Med 7: 657-662, 2014.
APA
Zhang, P., Huang, Z., Yan, H., Su, L., Gui, Y., Lv, H. ... Li, T. (2014). Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion. Experimental and Therapeutic Medicine, 7, 657-662. https://doi.org/10.3892/etm.2014.1483
MLA
Zhang, P., Huang, Z., Yan, H., Su, L., Gui, Y., Lv, H., Zhu, B., Li, T."Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion". Experimental and Therapeutic Medicine 7.3 (2014): 657-662.
Chicago
Zhang, P., Huang, Z., Yan, H., Su, L., Gui, Y., Lv, H., Zhu, B., Li, T."Improvement of the suture-occluded method in rat models of focal cerebral ischemia-reperfusion". Experimental and Therapeutic Medicine 7, no. 3 (2014): 657-662. https://doi.org/10.3892/etm.2014.1483