Differential expression of miRNA in rat myocardial tissues under psychological and physical stress

Cui,Yan; Bai,Yun; Wang,Xiao Dong; Liu,Bin; Zhao,Zhuo; Wang,Li Shuang

doi:10.3892/etm.2014.1504

Differential expression of miRNA in rat myocardial tissues under psychological and physical stress

Authors:
- Yan Cui
- Yun Bai
- Xiao Dong Wang
- Bin Liu
- Zhuo Zhao
- Li Shuang Wang
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Affiliations: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, P.R. China, Department of Endoscopy, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
Published online on: January 27, 2014 https://doi.org/10.3892/etm.2014.1504
Pages: 901-906

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Abstract

In the current study, microRNA (miRNA) microarrays were used to detect differentially expressed miRNAs in the myocardial tissues of rat models under stress, to screen target miRNA candidates for miRNA therapy of stress‑induced myocardial injury. Rats were bound and suspended in order to induce acute stress (AS) and chronic stress (CS) models. miRNA microarrays were used to detect differentially expressed miRNA in the myocardial tissues of the stressed and control groups. In comparison to the normal control, there were 68 differentially expressed miRNAs in the AS model, of which 32 were upregulated and 36 were downregulated. There were 55 differentially expressed miRNAs in the CS model, of which 20 were upregulated and 35 were downregulated. Of the 123 miRNAs, 15 miRNAs were differentially expressed between the AS and CS groups, of which four were significantly upregulated (rno‑miR‑296, rno‑miR‑141, rno‑miR‑382 and rno‑miR‑219‑5p) and 11 were downregulated (significantly downregulated, rno‑miR‑135a and rno‑miR‑466b). The stress of being bound and suspended induces myocardial injury in the rats. Myocardial injury may cause the differential expression of certain miRNAs. Psychological stress may lead to the significant upregulation of rno‑miR‑296, rno‑miR‑141, rno‑miR‑382 and rno‑miR‑219‑5p in addition to the significant downregulation of miR‑135a and miR‑466b.

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