Protective effects of hydrogen‑rich saline in uncontrolled hemorrhagic shock

Du,Zunmin; Jia,Haipeng; Liu,Jing; Zhao,Xiaomin; Wang,Yun; Sun,Xuejun

doi:10.3892/etm.2014.1572

Protective effects of hydrogen‑rich saline in uncontrolled hemorrhagic shock

Authors:
- Zunmin Du
- Haipeng Jia
- Jing Liu
- Xiaomin Zhao
- Yun Wang
- Xuejun Sun
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Affiliations: Department of Hematology, The 88th Hospital of Chinese People's Liberation Army, Tai'an, Shandong 271000, P.R. China, Atherosclerosis Research Institute of Taishan Medical University, Tai'an, Shandong 271000, P.R. China, Department of Diving Medicine, Faculty of Naval Medicine, The Second Military Medical University, Shanghai 200433, P.R. China
Published online on: February 21, 2014 https://doi.org/10.3892/etm.2014.1572
Pages: 1253-1258

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Abstract

Hydrogen is considered to be a novel antioxidant as it inhibits inflammation, removes oxygen‑derived free radicals and reduces oxidative damage. This study investigated the effects of hydrogen‑rich saline on plasma interleukin‑6 (IL‑6), tumor necrosis factor‑α (TNF‑α), superoxide dismutase (SOD) and malondialdehyde (MDA) in rats with uncontrolled hemorrhagic shock (UHS). The UHS model was induced by arterial bleeding and tail amputation. The rats were randomly divided into: Group A (sham‑operated group), Group B [shock + intravenously (IV) injected saline], Group C (shock + IV‑injected hydrogen‑rich saline), Group D [shock + intraperitoneally (IP) injected saline] and Group E (shock + IP‑injected hydrogen‑rich saline). The survival rate 24 h after successful resuscitation was calculated. The mean arterial pressure and heart rate were recorded at 0, 30, 90 and 210 min. The plasma levels of IL‑6, TNF‑α, SOD and MDA were measured at 0, 90 and 210 min. The survival rate of each group was 100% and the hemodynamics among the experimental groups were not significantly different. At 90 and 210 min, the levels of IL‑6, TNF‑α and MDA in Groups C and E were lower than those of Groups B and D, while the SOD levels were higher than those of Groups B and D (P<0.01). At 90 min, the levels of IL‑6, TNF‑α and MDA in Groups B and C were lower than those of Groups D and E, respectively (P<0.01). Hydrogen‑rich saline has anti‑inflammatory and anti‑oxidative effects in UHS. In conclusion, the results showed that itravenous injection of hydrogen-rich saline is more effective than intraperitonal injection.

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1	Huang CS, Kawamura T, Toyoda Y and Nakao A: Recent advances in hydrogen research as a therapeutic medical gas. Free Radic Res. 44:971–982. 2010. View Article : Google Scholar : PubMed/NCBI
2	Cai J, Kang Z, Liu WW, et al: Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model. Neurosci Lett. 441:167–172. 2008. View Article : Google Scholar : PubMed/NCBI
3	Zheng X, Mao Y, Cai J, et al: Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats. Free Radic Res. 43:478–484. 2009. View Article : Google Scholar : PubMed/NCBI
4	Cai J, Kang Z, Liu K, et al: Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model. Brain Res. 1256:129–137. 2009. View Article : Google Scholar : PubMed/NCBI
5	Liu W, Dong XS and Liu Z: Early liquid recovery combined 2% hydrogen suction to protection of lung injury induced by LPS to septic shock in rats. Jilin Medical Journal. 31:5939–5941. 2010.
6	Ohsawa I, Ishikawa M, Takahashi K, et al: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 13:688–694. 2007. View Article : Google Scholar
7	Mao YF, Zheng XF, Cai JM, et al: Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats. Biochem Biophys Res Commun. 381:602–605. 2009. View Article : Google Scholar : PubMed/NCBI
8	Capone AC, Safar P, Stezoski SW, et al: Improved outcome with fluid restriction in treatment of uncontrolled hemorrhagic shock. J Am Coll Surg. 180:49–56. 1995.PubMed/NCBI
9	Watters JM, Tieu BH, Todd SR, et al: Fluid resuscitation increases inflammatory gene transcription after traumatic injury. J Trauma. 61:300–308. 2006. View Article : Google Scholar : PubMed/NCBI
10	Alam HB and Rhee P: New developments in fluid resuscitation. Surg Clin North Am. 87:55–72. 2007. View Article : Google Scholar : PubMed/NCBI
11	Tracey KJ, Beutler B, Lowry SF, et al: Shock and tissue injury induced by recombinant human cachectin. Science. 234:470–474. 1986. View Article : Google Scholar : PubMed/NCBI
12	Sun Z, Wang X, Lasson A, et al: Roles of platelet-activating factor, interleukin-1beta and interleukin-6 in intestinal barrier dysfunction induced by mesenteric arterial ischemia and reperfusion. J Surg Res. 87:90–100. 1999. View Article : Google Scholar
13	Otamiri T: Oxygen radicals, lipid peroxidation, and neutrophil infiltration after small-intestinal ischemia and reperfusion. Surgery. 105:593–597. 1989.PubMed/NCBI
14	Qiu ZL, Wang ZJ, Zheng CM, et al: The effect of tumor necrosis factor-α monoclonal antibody in liver of rats on acute hemorrhagic shock. J Hepatopancreatobil Surg. 24:228–231. 2012.
15	Tan LR, Waxman K, Clark L, et al: Superoxide dismutase and allopurinol improve survival in animal model of the hemorrhagic shock. Am Surg. 59:797–800. 1993.PubMed/NCBI
16	Rhee P, Waxman K, Clark L, et al: Superoxide dismutase polyethylene glycol improves survival in hemorrhagic shock. Am Surg. 57:747–750. 1991.PubMed/NCBI
17	Du ZM, Jia HP, Liu J, et al: Experimental study of hydrogen-rich saline on plasma inflammatory and Oxidative damage factor in uncontrolled hemorrhagic shock in rats. Chin J Exp Surg. 30:24552013.(In Chinese).
18	Qian L, Cao F, Cui J, et al: Radio protective effect of hydrogen in cultured cells and mice. Free Radic Res. 44:275–282. 2010. View Article : Google Scholar : PubMed/NCBI
19	Xie K, Yu Y, Pei Y, et al: Protective effects of hydrogen gas on murine polymicrobial sepsis via reducing oxidative stress and HMGB1 release. Shock. 34:90–97. 2010. View Article : Google Scholar : PubMed/NCBI
20	Wang CY, Fu YB, Wang YL, et al: Role of direct peritoneal resuscitation in hemorrhagic shock in rats. Medical Journal of Wuhan University. 30:727–731. 2009.(In Chinese).
21	Xu Z, Zhou JR, Sun X and Jiang CL: The anti-inflammation effect of hydrogen saline on paw oedema. Prog Mod Biomed. 10:4421–4424. 2010.
22	Buchholz BM, Kaczorowski DJ, Sugimoto R, et al: Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant. 8:2015–2024. 2008. View Article : Google Scholar : PubMed/NCBI
23	Chen H, Sun YP, Hu PF, et al: The effects of hydrogen-rich saline on the contractile and structural changes of intestine induced by ischemia-reperfusion in rats. J Surg Res. 167:316–322. 2011. View Article : Google Scholar : PubMed/NCBI