Protective effects of the combination of sodium ferulate and oxymatrine on cecal ligation and puncture‑induced sepsis in mice

Xu,Mengxin; Wang,Wei; Pei,Xiaokun; Sun,Songmei; Xu,Mingbo; Liu,Zhifeng

doi:10.3892/etm.2014.1604

Protective effects of the combination of sodium ferulate and oxymatrine on cecal ligation and puncture‑induced sepsis in mice

Authors:
- Mengxin Xu
- Wei Wang
- Xiaokun Pei
- Songmei Sun
- Mingbo Xu
- Zhifeng Liu
View Affiliations

Affiliations: School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China, Beijing SL Pharmaceutical Co., Ltd., Beijing 100049, P.R. China
Published online on: March 5, 2014 https://doi.org/10.3892/etm.2014.1604
Pages: 1297-1304

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to investigate the effects of the combination of sodium ferulate (SF) and oxymatrine (OMT) on mice with cecal ligation and puncture (CLP)‑induced sepsis. Swiss male mice were randomly divided into a control group, CLP group, three SF + OMT groups (3.1+6.9; 6.2+13.8 and 12.3+27.7 mg/kg), SF (6.2 mg/kg) group and OMT (13.8 mg/kg) group. Eight hours after the administration of the drugs, the survival rates and survival times of the animals were monitored. In addition, the lung wet/dry weight (W/D) ratio; alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in the serum; the C‑reactive protein (CRP), interleukin‑6 (IL‑6) and interferon‑γ (IFN‑γ) levels in the serum and lung and liver homogenates; and the malondialdehyde (MDA) and superoxidase dismutase (SOD) levels in the lung and liver homogenates were measured. The bacterial load in the serum was also studied. Following treatment with the combination of SF and OMT, the survival rate increased and the survival time was prolonged; CLP‑induced increases in the lung W/D ratio and the levels of ALT, AST, LDH, CRP, IL‑6, IFN‑γ and MDA were significantly reduced; and the SOD activity levels were increased, compared with those of the untreated animals with CLP‑induced sepsis. These results indicated that the combination of SF and OMT induced protective effects against CLP‑induced lethal sepsis of mice. The possible mechanism of these effects may be associated with the alleviation of systemic inflammation and diminishment of oxidative injury.

View Figures

View References

1	Mokart D, Capo C, Blache JL, Delpero JR, Houvenaeghel G, Martin C and Mege JL: Early postoperative compensatory anti-inflammatory response syndrome is associated with septic complications after major surgical trauma in patients with cancer. Br J Surg. 89:1450–1456. 2002. View Article : Google Scholar
2	Bollaert PE, Charpentier C, Levy B, Debouverie M, Audibert G and Larcan A: Reversal of late septic shock with supraphysiologic doses of hydrocortisone. Crit Care Med. 26:645–650. 1998. View Article : Google Scholar : PubMed/NCBI
3	Baddour LM, Yu VL, Klugman KP, et al; International Pneumococcal Study Group. Combination antibiotic therapy lowers mortality among severely ill patients with pneumococcal bacteremia. Am J Respir Crit Care Med. 170:440–444. 2004. View Article : Google Scholar : PubMed/NCBI
4	Schlichting D and McCollam JS: Recognizing and managing severe sepsis: a common and deadly threat. South Med J. 100:594–600. 2007. View Article : Google Scholar : PubMed/NCBI
5	Yuan X, Sun Y, Miao N, et al: The synergistic anti-inflammatory effect of the combination of sodium ferulate and oxymatrine and its modulation on inflammation-associated mediators in RAW 264.7 cells. J Ethnopharmacol. 137:1477–1485. 2011. View Article : Google Scholar : PubMed/NCBI
6	Liu H, Sun Y, Gao Y, Chen F, Xu M and Liu Z: The analgesic effect and mechanism of the combination of sodium ferulate and oxymatrine. Neurochem Res. 35:1368–1375. 2010. View Article : Google Scholar : PubMed/NCBI
7	Yuan X, Wang Y, Du D, Hu Z, Xu M, Xu M and Liu Z: The effects of the combination of sodium ferulate and oxymatrine on lipopolysaccharide-induced acute lung injury in mice. Inflammation. 35:1161–1168. 2012. View Article : Google Scholar : PubMed/NCBI
8	Baker CC, Chaudry IH, Gaines HO and Baue AE: Evaluation of factors affecting mortality rate after sepsis in a murine cecal ligation and puncture model. Surgery. 94:331–335. 1983.PubMed/NCBI
9	Standage SW, Caldwell CC, Zingarelli B and Wong HR: Reduced peroxisome proliferator-activated receptor α expression is associated with decreased survival and increased tissue bacterial load in sepsis. Shock. 37:164–169. 2012.
10	Elstner EF and Heupel A: Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Anal Biochem. 70:616–620. 1976. View Article : Google Scholar : PubMed/NCBI
11	Ohkawa H, Ohishi N and Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95:351–358. 1979. View Article : Google Scholar : PubMed/NCBI
12	Li Y, Huang Y, Piao Y, Nagaoka K, Watanabe G, Taya K and Li C: Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis. Reprod Biol Endocrinol. 11:232013. View Article : Google Scholar : PubMed/NCBI
13	Wei Q, Ren X, Jiang Y, Jin H, Liu N and Li J: Advanced glycation end products accelerate rat vascular calcification through RAGE/oxidative stress. BMC Cardiovasc Disord. 13:132013. View Article : Google Scholar : PubMed/NCBI
14	Wang BH and Ou-Yang JP: Pharmacological actions of sodium ferulate in cardiovascular system. Cardiovascuar Drug Rev. 23:161–172. 2005. View Article : Google Scholar : PubMed/NCBI
15	Srinivasan M, Sudheer AR and Menon VP: Ferulic acid: therapeutic potential through its antioxidant property. J Clin Biochem Nutr. 40:92–100. 2007. View Article : Google Scholar : PubMed/NCBI
16	Chen XS, Wang GJ, Cai X, et al: Inhibition of hepatitis B virus by oxymatrine in vivo. World J Gastroenterol. 7:49–52. 2001.PubMed/NCBI
17	Xu GL, Yao L, Rao SY, Gong ZN, Zhang SQ and Yu SQ: Attenuation of acute lung injury in mice by oxymatrine is associated with inhibition of phosphorylated p53 mitogen-activated protein kinase. J Ethnopharmacol. 98:177–183. 2005. View Article : Google Scholar : PubMed/NCBI
18	Brooks HF, Osabutey CK, Moss RF, Andrews PL and Davies DC: Caecal ligation and puncture in the rat mimics the pathophysiological changes in human sepsis and causes multiorgan dysfunction. Metab Brain Dis. 22:353–373. 2007. View Article : Google Scholar : PubMed/NCBI
19	Remick D, Manohar P, Bolgos G, Rodriguez J, Moldawer L and Wollenberg G: Blockade of tumor necrosis factor reduces lipopolysacharide lethality, but not the lethality of cecal ligation and puncture. Shock. 4:89–95. 1995. View Article : Google Scholar : PubMed/NCBI
20	Kosaka J, Morimatsu H, Takahashi T, et al: Effects of biliverdin administration on acute lung injury induced by hemorrhagic shock and resuscitation in rats. PLoS One. 8:e636062013. View Article : Google Scholar : PubMed/NCBI
21	Shahshahani MM, Azizahari S, Soori T, et al: Hepatotoxicity and liver enzyme alteration in patients with immunobullous diseases receiving immunosuppressive therapy. J Dermatol. 38:1153–1157. 2011. View Article : Google Scholar
22	Singab AN, Youssef DT, Noaman E and Kotb S: Hepatoprotective effect of flavonol glycosides rich fraction from Egyptian Vicia calcarata Desf. against CCl₄-induced liver damage in rats. Arch Pharm Res. 28:791–798. 2005. View Article : Google Scholar : PubMed/NCBI
23	Márquez-Velasco R, Martínez-Velázquez AX, Amezcua-Guerra LM, et al: Enhanced survival from CLP-induced sepsis following late administration of low doses of anti-IFNγ F(ab’)2 antibody fragments. Inflamm Res. 60:947–953. 2011.PubMed/NCBI
24	Latifi SQ, O’Riordan MA, Levine AD and Stallion A: Persistent elevation of serum interleukin-6 in intraabdominal sepsis identifies those with prolonged length of stay. J Pediatr Surg. 39:1548–1552. 2004. View Article : Google Scholar : PubMed/NCBI
25	Imamura T, Tanaka S, Yoshida H, et al: Significance of measurement of high sensitivity C-reactive protein in acute pancreatitis. J Gastroenterol. 37:935–938. 2002. View Article : Google Scholar : PubMed/NCBI
26	Memiş D, Gursoy O, Tasdogan M, et al: High C-reactive protein and low cholesterol levels are prognostic markers of survival in severe sepsis. J Clin Anesth. 19:186–191. 2007.PubMed/NCBI
27	Ballou SP and Lozanski G: Induction of inflammatory cytokine release from cultured human monocytes by C-reactive protein. Cytokine. 4:361–368. 1992. View Article : Google Scholar : PubMed/NCBI
28	Cermak J, Key NS, Bach RR, Balla J, Jacob HS and Vercellotti GM: C-reactive protein induces human peripheral blood monocytes to synthesize tissue factor. Blood. 82:513–520. 1993.PubMed/NCBI
29	Lausevic Z, Lausevic M, Trbojevic-Stankovic J, Krstic S and Stojimirovic B: Predicting multiple organ failure in patients with severe trauma. Can J Surg. 51:97–102. 2008.PubMed/NCBI
30	Calandra T, Gerain J, Heumann D, et al: High circulating levels of interleukin-6 in patients with septic shock: Evolution during sepsis, prognostic value, and interplay with other cytokines. The Swiss-Dutch J5 Immunoglobulin Study Group. Am J Med. 91:23–29. 1991. View Article : Google Scholar : PubMed/NCBI
31	Gårdlund B, Sjölin J, Nilsson A, et al: Plasma levels of cytokines in primary septic shock in humans: correlation with disease severity. J Infect Dis. 172:296–301. 1995.PubMed/NCBI
32	Coyle P, Philcox JC and Rofe AM: Metallothionein induction in cultured rat hepatocytes by arthritic rat serum, activated macrophages, interleukin-6, interleukin-11 and leukaemia inhibitory factor. Inflamm Res. 44:475–481. 1995. View Article : Google Scholar
33	Tao JY, Zheng GH, Zhao L, et al: Anti-inflammatory effects of ethyl acetate fraction from Melilotus suaveolens Ledeb on LPS-stimulated RAW 264.7 cells. J Ethnopharmacol. 123:97–105. 2009. View Article : Google Scholar : PubMed/NCBI
34	de Lima TH, Sass N, Mattar R, Moron AF, Torloni MR, Franchim CS and Daher S: Cytokine gene polymorphisms in preeclampsia and eclampsia. Hypertens Res. 32:565–569. 2009.PubMed/NCBI
35	Su F, Wang Z, Cai Y, Remmelink M and Vincent JL: Beneficial effects of ethyl pyruvate in septic shock from peritonitis. Arch Surg. 142:166–171. 2007. View Article : Google Scholar : PubMed/NCBI
36	Berg RM, Møller K and Bailey DM: Neuro-oxidative-nitrosative stress in sepsis. J Cereb Blood Flow Metab. 31:1532–1544. 2011. View Article : Google Scholar : PubMed/NCBI
37	Perše M, Injac R and Erman A: Oxidative status and lipofuscin accumulation in urothelial cells of bladder in aging mice. PLoS One. 8:e596382013.PubMed/NCBI
38	Gong WH, Zheng WX, Wang J, Chen SH, Pang B, Hu XM and Cao XL: Coexistence of hyperlipidemia and acute cerebral ischemia/reperfusion induces severe liver damage in a rat model. World J Gastroenterol. 18:4934–4943. 2012. View Article : Google Scholar : PubMed/NCBI
39	Fakurazi S, Sharifudin SA and Arulselvan P: Moringa oleifera hydroethanolic extracts effectively alleviate acetaminophen-induced hepatotoxicity in experimental rats through their antioxidant nature. Molecules. 17:8334–8350. 2012. View Article : Google Scholar
40	Sunil C, Duraipandiyan V, Ignacimuthu S and Al-Dhabi NA: Antioxidant, free radical scavenging and liver protective effects of friedelin isolated from Azima tetracantha Lam. leaves. Food Chem. 139:860–865. 2013. View Article : Google Scholar : PubMed/NCBI