Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes

Ang,Hui  Ying; Subramani,Tamilselvan; Yeap,Swee  Keong; Omar,Abdul  Rahman; Ho,Wan  Yang; Abdullah,Mohd  Puad; Alitheen,Noorjahan  Banu

doi:10.3892/etm.2014.1657

June-2014 Volume 7 Issue 6

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June-2014 Volume 7 Issue 6

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Article

Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes

Authors:
- Hui Ying Ang
- Tamilselvan Subramani
- Swee Keong Yeap
- Abdul Rahman Omar
- Wan Yang Ho
- Mohd Puad Abdullah
- Noorjahan Banu Alitheen
View Affiliations / Copyright

Affiliations: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia, The University of Nottingham Malaysia Campus, Semenyih, Selangor 43500, Malaysia
Pages: 1733-1737
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Published online on: April 2, 2014

https://doi.org/10.3892/etm.2014.1657
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Abstract

Immunomodulators are agents that are able to stimulate or inhibit the immune response. The leaf extracts from Potentilla indica and Dendrophthoe pentandra were analyzed in vitro for immunomodulatory activity and an MTT colorimetric assay was conducted to determine the proliferation of mice splenocytes and thymocytes. A bromodeoxyuridine assay was performed to analyze DNA synthesis and the Trypan blue exclusion method was conducted to evaluate the changes in total cell population. The results indicated that treatment with P. indica and D. pentandra produced a time‑ and dose-dependent increase in cell viability and proliferation. Following 72 h of treatment with P. indica and D. pentandra, thymocyte proliferation was augmented by 18 and 41%, respectively and splenocyte proliferation increased by 35 and 42%, respectively, when compared with untreated cells. The present study demonstrated that these extracts may act as potential immunostimulants and, thus, represent an alternative source of immunomodulatory compounds for the treatment of human immune‑mediated diseases.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes

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