Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO‑cGMP signaling pathway

Yin,Yaling; Wan,Jia; Li,Peng; Jia,Yanlong; Sun,Ruili; Pan,Guopin; Wan,Guangrui

doi:10.3892/etm.2014.1700

Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO‑cGMP signaling pathway

Authors:
- Yaling Yin
- Jia Wan
- Peng Li
- Yanlong Jia
- Ruili Sun
- Guopin Pan
- Guangrui Wan
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Affiliations: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 45300, P.R. China, Department of Nephrology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, College of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Inspection, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Modern Technology Education Center, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Published online on: May 2, 2014 https://doi.org/10.3892/etm.2014.1700
Pages: 38-48

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Abstract

The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule‑1, vascular adhesion molecule‑1, interleukin‑1 and ‑6 and nuclear factor‑κB decreased, while the expression levels of matrix metalloproteinase‑2 and tissue inhibitor of metalloproteinase‑2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti‑inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2‑induced injury of HUVECs, which may be associated with the NO‑cyclic guanosine monophosphate signaling pathway.

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