Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice

Chen,Shaocheng; Zhu,Kai; Wang,Rui; Zhao,Xin

doi:10.3892/etm.2014.1712

Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice

Authors:
- Shaocheng Chen
- Kai Zhu
- Rui Wang
- Xin Zhao
View Affiliations

Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
Published online on: May 14, 2014 https://doi.org/10.3892/etm.2014.1712
Pages: 316-322

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In the present study the preventive effect of polysaccharides from the large yellow croaker swim bladder (PLYCSB) on HCl/ethanol‑induced gastric injury in ICR mice was investigated. A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)‑1β, IL‑6, IL‑8, as well as increase the levels of IL‑4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice. The somatostatin and vasoactive intestinal peptide serum levels in PLYCSB‑treated mice were higher compared with those in control mice, whilst motilin and substance P serum levels were lower compared with those in control mice. The extent of the gastric injury in the mice treated with PLYCSB was lower compared with that in the control mice; however, the results obtained for mice treated with a high dose of PLYCSB were similar to those for omeprazole‑treated mice. In addition, the superoxide dismutase and glutathione peroxidase activities of PLYCSB‑treated mice were higher compared with those of the control mice, and similar to those observed in normal and omeprazole‑treated mice. Furthermore, PLYCSB‑treated mice showed levels of nitric oxide and malondialdehyde that were similar to those in the normal group. Using PCR and western blot analysis, it was demonstrated that PLYCSB significantly inhibited inflammation in the tissues of the HCl/ethanol induced gastric injury mice by downregulating the expression of inducible nitric oxide synthase, cyclooxygenase‑2, tumor necrosis factor‑α and IL‑1β. These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole. Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.

View Figures

View References

1	Jian JC and Wu ZH: Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture. 218:1–9. 2003. View Article : Google Scholar
2	Li C and Yao CL: Molecular and expression characterizations of interleukin-8 gene in large yellow croaker (Larimichthys crocea). Fish Shellfish Immunol. 34:799–809. 2013. View Article : Google Scholar : PubMed/NCBI
3	Liu S and Yu B: Peptides from variegated carp (Aristichthys nobilis) swim bladder: Fermentation production and assessment of antioxidant properties. Food Sci. 30:332–334. 2009.(In Chinese).
4	Yu ZH, Yin LH, Yang Q and Liu Y: Effect of Lentinus edodes polysaccharide on oxidative stress, immunity activity and oral ulceration of rats stimulated by phenol. Carbohydr Polym. 75:115–118. 2009.
5	Laurienzo P: Marine polysaccharides in pharmaceutical applications: an overview. Mar Drugs. 8:2435–2465. 2010. View Article : Google Scholar : PubMed/NCBI
6	Szabo S, Trier JS, Brown A and Schnoor J: Early vascular injury and increased vascular permeability in gastric mucosal injury caused by ethanol in the rat. Gastroenterology. 88:228–236. 1985. View Article : Google Scholar : PubMed/NCBI
7	Medeiros JV, Gadelha GG, Lima SJ, Garcia JA, Soares PM, Santos AA, Brito GA, Ribeiro RA and Souza MH: Role of the NO/cGMP/K(ATP) pathway in the protective effects of sildenafil against ethanol-induced gastric damage in rats. Br J Pharmacol. 153:721–727. 2008. View Article : Google Scholar : PubMed/NCBI
8	Yan C and Yan X: Study on extraction of Lycium barbarum polysaccharides by different methods and their antioxidant effects in vitro. Food Sci. 29:183–187. 2008.(In Chinese).
9	Ramirez RO and Roa CC Jr: The gastroprotective effect of tannins extracted from duhat (Syzygium cumini Skeels) bark on HCl/ethanol induced gastric mucosal injury in Sprague-Dawley rats. Clin Hemorheol Microcirc. 29:253–261. 2003.PubMed/NCBI
10	Wang Q, Zhao X, Qian Y and Wang R: In vitro antioxidative activity of yellow tea and its in vivo preventive effect on gastric injury. Exp Ther Med. 6:423–426. 2013.PubMed/NCBI
11	Zhao X, Kim SY and Park KY: Bamboo salt has in vitro anticancer activity in HCT-116 cells and exerts anti-metastatic effects in vivo. J Med Food. 16:9–19. 2013. View Article : Google Scholar : PubMed/NCBI
12	Cao H, Tian XL and Liu X: Study on molecular identification and pharmacology of hemostasis action for isinglass. Zhongguo Shipin Xuebao. 9(4): 170–176. 2009.(In Chinese).
13	Ligumsky M, Sestieri M, Okon E and Ginsburg I: Antioxidants inhibit ethanol-induced gastric injury in the rat. Role of manganese, glycine, and carotene. Scand J Gastroenterol. 30:854–860. 1995. View Article : Google Scholar : PubMed/NCBI
14	Gratacós J, Collado A, Filella X, Sanmartí R, Cañete J, Llena J, Molina R, Ballesta A and Muñoz-Gómez J: Serum cytokines (IL-6, TNF-alpha, IL-1 beta and IFN-gamma) in ankylosing spondylitis: a close correlation between serum IL-6 and disease activity and severity. Br J Rheumatol. 33:927–931. 1994.PubMed/NCBI
15	Fox JG, Beck P, Dangler CA, Whary MT, Wang TC, Shi NH and Nagler-Anderson C: Concurrent enteric helminth infection modulates inflammation and gastric immune responses and reduces helicobacter-induced gastric atrophy. Nat Med. 6:536–542. 2000. View Article : Google Scholar
16	D’Elios MM, Manghetti M, De Carli M, Costa F, Baldari CT, Burroni D, Telford JL, Romagnani S and Del Prete G: T helper 1 effector cells specific for Helicobacter pylori in the gastric antrum of patients with peptic ulcer disease. J Immunol. 158:962–967. 1997.
17	Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB and Ruddle FH: Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21. Genomics. 2:203–208. 1988. View Article : Google Scholar : PubMed/NCBI
18	van der Poll T, Keogh CV, Guirao X, Buurman WA, Kopf M and Lowry SF: Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia. J Infect Dis. 176:439–444. 1997.PubMed/NCBI
19	Maeda S and Yanagihara Y: Inflammatory cytokines (IL-4, IL-5 and IL-13). Nippon Rinsho. 59:1894–1899. 2001.(In Japanese).
20	Vlahopoulos S, Boldogh I, Casola A and Brasier AR: Nuclear factor-kappaB-dependent induction of interleukin-8 gene expression by tumor necrosis factor alpha: evidence for an antioxidant sensitive activating pathway distinct from nuclear translocation. Blood. 94:1878–1889. 1999.
21	Wang HY, Liu YM, Li HY, Feng QJ, Guo JY and Niu X: Effects of oils in Alpinia officinarum Hance on serum motilin, somatostatin, substance P, vasoactive intestinal peptide in gastrelcosis mice model. Chinese J Exp Tradit Med Formulae. 17(4): 105–107. 2011.(In Chinese).
22	Zhang SR, Shao JY and Yu YW: The protective effects of furazolidone and some commonly used antiulcer drugs on several gastric ulcer models in rats. Yao Xue Xue Bao. 19:5–11. 1984.(In Chinese).
23	Zhao X, Wang Q, Qian Y and Song JL: Ilex kudingcha C.J. Tseng (Kudingcha) prevents HCl/ethanol-induced gastric injury in Sprague-Dawley rats. Mol Med Rep. 7:1613–1616. 2013.PubMed/NCBI
24	Qu CY, Li DG, Wang YQ and Chen MM: Effect of chitosan on the serum levels of MDA, SOD, and GSH-Px in rats with gastric ulcer. Shanghai Med Pharm J. 29:219–222. 2008.(In Chinese).
25	Ligumsky M, Sestieri M, Okon E and Ginsburg I: Antioxidants inhibit ethanol-induced gastric injury in the rat: Role of manganese, glycine, and carotene. Scand J Gastroenterol. 30:854–860. 1995. View Article : Google Scholar : PubMed/NCBI
26	Cheng HH and AXR: Change of serum nitrogen monoxidum and nitrogen monoxidum synthase levels in patients with peptic ulcer in high altitude region. Lab Med. 25:427–428. 2010.(In Chinese).
27	Wang G, Tu ZL, Chen L, Yuan SH and Yang GY: Mechanism of antiulcer effects of Jinguolan. Herald Med. 28:42–45. 2009.(In Chinese).
28	Li HL, Sun BZ and Ma FC: Expression of COX-2, iNOS, p53 and Ki-67 in gastric mucosa-associated lymphoid tissue lymphoma. World J Gastroenterol. 10:1862–1866. 2004.PubMed/NCBI
29	Hayden MS and Ghosh S: Signaling to NF-kappaB. Gene Dev. 18:2195–2224. 2004. View Article : Google Scholar : PubMed/NCBI
30	Kim SF, Huri DA and Snyder SH: Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science. 310:1966–1970. 2005. View Article : Google Scholar : PubMed/NCBI