Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma

Yang,Yang; Hui,Lv; Yuqin,Che; Jie,Li; Shuai,Hou; Tiezhu,Zhou; Wei,Wang

doi:10.3892/etm.2014.1756

Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma

Authors:
- Yang Yang
- Lv Hui
- Che Yuqin
- Li Jie
- Hou Shuai
- Zhou Tiezhu
- Wang Wei
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Affiliations: Department of Neurology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China
Published online on: June 4, 2014 https://doi.org/10.3892/etm.2014.1756
Pages: 563-566

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Abstract

Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3‑kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logarithmic growth phase were seeded into six‑well plates at a density of 104 cells/well. In the experimental group, 1 µl/ml saw palmetto extract was added, while the control group was cultured without a drug for 24 h. The expression levels of PI3K, B‑cell lymphoma‑extra large (Bcl‑xL) and p53 were evaluated through western blot analysis. In the experimental group, the U87 and U251 cells exhibited a lower expression level of PI3K protein as compared with the control group (t=6.849; P<0.001). In addition, the two cell lines had a higher expression level of p53 protein in the experimental group as compared with the control group (t=40.810; P<0.001). Protein expression levels of Bcl‑xL decreased significantly in the experimental group as compared with the control group (t=19.640; P=0.000). Therefore, saw palmetto extract induces glioma cell growth arrest and apoptosis via decreasing PI3K/Akt signal transduction.

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1	Ohgaki H and Kleihues P: Epidemiology and etiology of gliomas. Acta Neuropathol. 109:93–108. 2005. View Article : Google Scholar : PubMed/NCBI
2	Wen PY and Kesari S: Malignant gliomas in adults. N Engl J Med. 359:492–507. 2008. View Article : Google Scholar : PubMed/NCBI
3	Jane EP, Premkumar DR, Morales A, Foster KA and Pollack IF: Inhibition of phosphatidylinositol 3-kinase/AKT signaling by NVP-BKM120 promotes ABT-737-induced toxicity in a caspase-dependent manner through mitochondrial dysfunction and DNA damage response in established and primary cultured glioblastoma cells. J Pharmacol Exp Ther. 349:1–60. 2014.
4	Castellino RC and Durden DL: Mechanisms of disease: the P13K-Akt-PTEN signaling node - an intercept point for the control of angiogenesis in brain tumors. Nat Clin Pract Neurol. 3:682–693. 2007. View Article : Google Scholar : PubMed/NCBI
5	Roell D, Rösler TW, Degen S, Matusch R and Baniahmad A: Antiandrogenic activity of anthranilic acid ester derivatives as novel lead structures to inhibit prostate cancer cell proliferation. Chem Biol Drug Des. 77:450–459. 2011. View Article : Google Scholar : PubMed/NCBI
6	Smirnova ZS, Ermakova KV, Kubasova IY, Borisova LM, Kiselyova MP, Oborotova NA, Meerovich GA and Luk’yanets EA: Experimental study of combined therapy for malignant glioma. Bull Exp Biol Med. 156:480–482. 2014. View Article : Google Scholar : PubMed/NCBI
7	Nagane M: Anti-angiogenic therapy for malignant glioma. Gan To Kagaku Ryoho. 41:141–147. 2014.(In Japanese).
8	Taraphdar AK, Madhumita R and Bhattacharya RK: Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Curr Sci. 80:1387–1396. 2001.
9	Petrangeli E, Lenti L, Buchetti B, et al: Lipido-sterolic extract of Serenoa repens (LSESr, Permixon) treatment affects human prostate cancer cell membrane organization. J Cell Physiol. 219:69–76. 2009.
10	Chua T, Eise NT, Simpson JS and Ventura S: Pharmacological characterization and chemical fractionation of a liposterolic extract of saw palmetto (Serenoa repens): effects on rat prostate contractility. J Ethnopharmacol. 152:283–291. 2014. View Article : Google Scholar : PubMed/NCBI
11	Penugonda K and Lindshield BL: Fatty acid and phytosterol content of commercial saw palmetto supplements. Nutrients. 5:3617–3633. 2013. View Article : Google Scholar : PubMed/NCBI
12	Yang Y, Ikezoe T, Zheng Z, Taguchi H, Koeffler HP and Zhu WG: Saw palmetto induces growth arrest and apoptosis of androgen-dependent prostate cancer LNCaP cells via inactivation of STAT 3 and androgen receptor signaling. Int J Oncol. 31:593–600. 2007.PubMed/NCBI
13	Ding D, Wei S, Song Y, Li L, Du G, Zhan H and Cao Y: Osthole exhibits anti-cancer property in rat glioma cells through inhibiting PI3K/Akt and MAPK signaling pathways. Cell Physiol Biochem. 32:1751–1760. 2013. View Article : Google Scholar : PubMed/NCBI
14	Vitucci M, Karpinich NO, Bash RE, et al: Cooperativity between MAPK and PI3K signaling activation is required for glioblastoma pathogenesis. Neuro Oncol. 15:1317–1329. 2013. View Article : Google Scholar : PubMed/NCBI
15	Wei Y, Jiang Y, Zou F, et al: Activation of PI3K/Akt pathway by CD133-p85 interaction promotes tumorigenic capacity of glioma stem cells. Proc Natl Acad Sci USA. 110:6829–6834. 2013. View Article : Google Scholar : PubMed/NCBI
16	Barnholtz-Sloan JS, Sloan AE and Schwartz AG: Relative survival rates and patterns of diagnosis analyzed by time period for individuals with primary malignant brain tumor, 1973–1997. J Neurosurg. 99:458–466. 2003.PubMed/NCBI
17	Silvestri I, Cattarino S, Aglianò A, et al: Effect of Serenoa repens (Permixon(R)) on the expression of inflammation-related genes: analysis in primary cell cultures of human prostate carcinoma. J Inflamm (Lond). 10:112013.
18	Akl H, Vervloessem T, Kiviluoto S, Bittremieux M, Parys JB, De Smedt H and Bultynck G: A dual role for the anti-apoptotic Bcl-2 protein in cancer: Mitochondria versus endoplasmic reticulum. Biochim Biophys Acta. 14:S0167–S4889. 2014.PubMed/NCBI
19	Kerr JF, Wyllie AH and Currie AR: Apoptosis a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 26:239–257. 1972. View Article : Google Scholar : PubMed/NCBI
20	Los M, Burek CJ, Stroh C, et al: Anticancer drugs of tomorrow: apoptotic pathways as targets for drug design. Drug Discov Today. 8:67–77. 2003. View Article : Google Scholar : PubMed/NCBI
21	Yu Q, Li Y, Mu K, Li Z, Meng Q, Wu X, Wang Y and Li L: Amplification of Mdmx and overexpression of MDM2 contribute to mammary carcinogenesis by substituting for p53 mutations. Diagn Pathol. 9:1–8. 2014.PubMed/NCBI
22	Han L, Zhang AL, Xu P, et al: Combination gene therapy with PTEN and EGFR siRNA suppresses U251 malignant glioma cell growth in vitro and in vivo. Med Oncol. 27:843–852. 2010. View Article : Google Scholar : PubMed/NCBI
23	Choi JW, Schroeder MA, Sarkaria JN and Bram RJ: Cyclophilin B supports Myc and mutant p53-dependent survival of glioblastoma multiforme cells. Cancer Res. 74:484–496. 2014. View Article : Google Scholar : PubMed/NCBI
24	Gonzalez J and de Groot J: Combination therapy for malignant glioma based on PTEN status. Expert Rev Anticancer Ther. 8:1767–1779. 2008. View Article : Google Scholar : PubMed/NCBI