miR‑145 downregulates the expression of cyclin‑dependent kinase 6 in human cervical carcinoma cells

Zhang,Jing; Wang,Lu; Li,Baoli; Huo,Manpeng; Mu,Mingtao; Liu,Junjun; Han,Jiming

doi:10.3892/etm.2014.1765

miR‑145 downregulates the expression of cyclin‑dependent kinase 6 in human cervical carcinoma cells

Authors:
- Jing Zhang
- Lu Wang
- Baoli Li
- Manpeng Huo
- Mingtao Mu
- Junjun Liu
- Jiming Han
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Affiliations: Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi 716000, P.R. China
Published online on: June 6, 2014 https://doi.org/10.3892/etm.2014.1765
Pages: 591-594

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Abstract

The present study aimed to investigate the effect of the inhibition of miR‑145 on cyclin‑dependent protein kinase 6 (CDK6) and the proliferation of human cervical carcinoma cells. The miR‑145 sequence was synthesized and cloned into pcDNA™6.2‑GW to construct the recombinant plasmid pcDNA6.2‑GW‑miR‑145. HeLa cells were divided into the micro (mi)R‑145, normal control and blank groups. The transcription levels of miR‑145 and CDK6 were detected using quantitative polymerase chin reaction and western blot analysis was used to examine the CDK6 protein expression. In addition, the inhibitory effect of miR‑145 on the proliferation of HeLa cells was measured by an MTT assay. The recombinant plasmid pcDNA6.2‑GW‑miR‑145 was successfully constructed and used to transfect the HeLa cells in the MiR-145 group. The miR‑145 expression level in the miR‑145 group was significantly higher than that in the blank group. The CDK6 expression level in miR‑145 group was significantly lower than that in the blank group. Furthermore, miR‑145 inhibited the proliferation of HeLa cells. In conclusion, miR‑145 overexpression suppresses the expression of CDK6 and inhibits the proliferative ability of HeLa cells.

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