Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis

  • Authors:
    • Eun Jeong Choi
    • Jae Yeon Jung
    • Gun-Hee Kim
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  • Published online on: June 10, 2014     https://doi.org/10.3892/etm.2014.1771
  • Pages: 454-458
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Abstract

The present study investigated the effect of the phytochemical genistein on the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of estrogen receptor-α (ERα) expression and the induction of apoptosis. When MCF-7 human breast cancer cells were treated with 50, 100, 150 and 200 µM genistein for 24, 48 or 72 h, cell growth was significantly decreased in a concentration-dependent manner. Notably, the patterns of ERα expression and proliferation in MCF-7 cells treated with genistein were similar. Furthermore, ERα expression in differentiating 3T3-L1 cells was significantly inhibited by 48 h treatment with 50 µM genistein, which was selected based on the results of cytotoxicity assays on 3T3-L1 preadipocytes [lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol‑2-yl)‑2,5-diphenyltetrazolium bromide (MTT) viability assays]. Under the same conditions, genistein‑induced apoptotic features were observed in MCF-7 and differentiating 3T3-L1 cells. This observation is supported by the finding that B-cell lymphoma 2 (Bcl-2) expression was reduced while that of Bcl-2-associated X protein (Bax) was induced by genistein. The results of the present study suggest that an ERα-related pathway and the induction of apoptosis are involved in the proliferation of MCF-7 cells and the differentiation of 3T3-L1 cells.
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August-2014
Volume 8 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Choi EJ, Jung JY and Kim G: Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis. Exp Ther Med 8: 454-458, 2014.
APA
Choi, E.J., Jung, J.Y., & Kim, G. (2014). Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis. Experimental and Therapeutic Medicine, 8, 454-458. https://doi.org/10.3892/etm.2014.1771
MLA
Choi, E. J., Jung, J. Y., Kim, G."Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis". Experimental and Therapeutic Medicine 8.2 (2014): 454-458.
Chicago
Choi, E. J., Jung, J. Y., Kim, G."Genistein inhibits the proliferation and differentiation of MCF-7 and 3T3-L1 cells via the regulation of ERα expression and induction of apoptosis". Experimental and Therapeutic Medicine 8, no. 2 (2014): 454-458. https://doi.org/10.3892/etm.2014.1771