Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells

  • Authors:
    • Jun‑Feng Liu
    • Zhong‑Dong Du
    • Zhi Chen
    • Zhi‑Xu He
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  • Published online on: July 4, 2014     https://doi.org/10.3892/etm.2014.1827
  • Pages: 805-812
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Abstract

The number and function of endothelial progenitor cells (EPCs) may be a predictive factor for the severity and outcome of cardiovascular disease. However, the manipulation of bone marrow mononuclear cell (BMMC) cultures for EPCs is an elaborate and difficult procedure in small experimental animals. The present study aimed to assess the feasibility of whole bone marrow cell (WBMC) culture for expanding EPCs in small experimental animals. C57BL/6 mice (age, 3‑4 weeks; weight, 9.47±0.76 g) were used as the experimental animals, and WBMCs were isolated from the femora and tibiae and cultured in endothelial cell growth medium‑2. A BMMC culture for EPCs was used as a control. EPC growth, phenotype and functions were assessed in vitro and in vivo. The results demonstrated that EPCs were easily obtained from a WBMC culture in vitro. The cells exhibited similar growth and biological characteristics when compared with the EPCs derived from the traditional BMMC culture system. Thus, the cells were able to simultaneously bind to lectin and cause phagocytosis of acetylated-low density lipoproteins. In addition, the cells exhibited high expression levels of cluster of differentiation 34 and fetal liver kinase 1, and possessed similar functional properties to BMMC‑derived EPCs, including vascular network formation, proliferation, adhesion and migration abilities in vitro. Thus, WBMC‑derived EPCs can improve the outcome of pulmonary vascular disease when transplanted into a monocrotaline‑induced pulmonary hypertension mouse model. The results of the present study indicated that the WBMC culture system is a more convenient and effective method of obtaining and expanding EPCs compared with BMMC culture, with the advantage of a simplified procedure.
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September-2014
Volume 8 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu JF, Du ZD, Chen Z and He ZX: Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells. Exp Ther Med 8: 805-812, 2014.
APA
Liu, J., Du, Z., Chen, Z., & He, Z. (2014). Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells. Experimental and Therapeutic Medicine, 8, 805-812. https://doi.org/10.3892/etm.2014.1827
MLA
Liu, J., Du, Z., Chen, Z., He, Z."Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells". Experimental and Therapeutic Medicine 8.3 (2014): 805-812.
Chicago
Liu, J., Du, Z., Chen, Z., He, Z."Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells". Experimental and Therapeutic Medicine 8, no. 3 (2014): 805-812. https://doi.org/10.3892/etm.2014.1827