Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro

Shan,Ren  Feng; Zhou,Yun  Fei; Peng,Ai  Fen; Jie,Zhi  Gang

doi:10.3892/etm.2014.1844

Inhibition of Aurora-B suppresses HepG2 cell invasion and migration via the PI3K/Akt/NF-κB signaling pathway in vitro

Authors:
- Ren Feng Shan
- Yun Fei Zhou
- Ai Fen Peng
- Zhi Gang Jie
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Affiliations: Department of General Surgery, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, College of Humanity, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, P.R. China
Published online on: July 14, 2014 https://doi.org/10.3892/etm.2014.1844
Pages: 1005-1009

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Abstract

In the present study, the effect of Aurora‑B inhibition on HepG2 cell invasion and migration in vitro was investigated. A recombinant plasmid targeting the Aurora‑B gene (MiR‑Aurora‑B) was used to inhibit Aurora‑B expression in HepG2 cells. Cell migration and invasion were investigated using Transwell migration and invasion assays. The results demonstrated that cell invasion and migration were suppressed by inhibiting Aurora‑B. In addition, the effect of Aurora‑B inhibition on the activity of the phosphoinositide 3‑kinase (PI3K)/Akt/nuclear factor (NF)‑κB signaling pathway was investigated by analyzing the protein expression levels of phosphorylated (p)‑Akt, Akt, NF‑κB p65, matrix metalloproteinase (MMP)‑2 and MMP‑9 using western blot analysis. The results demonstrated that the protein expression levels of p‑Akt, NF‑κB p65, MMP‑2 and MMP‑9 were reduced significantly by inhibiting Aurora‑B. Therefore, inhibition of Aurora‑B was shown to suppress hepatocellular carcinoma cell migration and invasion by decreasing the activity of the PI3K/Akt/NF‑κB signaling pathway in vitro.

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