Regulatory effect of microRNA‑135a on the Th1/Th2 imbalance in a murine model of allergic rhinitis

Luo,Yanyun; Deng,Yuqin; Tao,Zezhang; Chen,Shiming; Xiao,Bokui; Ren,Jie; Chen,Zhe; Han,Jibo; Kong,Yonggang; Xu,Yu; Deng,Minjie

doi:10.3892/etm.2014.1855

Regulatory effect of microRNA‑135a on the Th1/Th2 imbalance in a murine model of allergic rhinitis

Authors:
- Yanyun Luo
- Yuqin Deng
- Zezhang Tao
- Shiming Chen
- Bokui Xiao
- Jie Ren
- Zhe Chen
- Jibo Han
- Yonggang Kong
- Yu Xu
- Minjie Deng
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Affiliations: Department of Otolaryngology ‑ Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: July 21, 2014 https://doi.org/10.3892/etm.2014.1855
Pages: 1105-1110

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Abstract

Allergic rhinitis (AR) is primarily caused by a T helper cell (Th)1/Th2 imbalance. In a murine AR model of a previous study, the serum ovalbumin (OVA)‑sIgE concentration was high, whereas microRNA (miR)‑135a was lowly expressed in the nasal mucosa. The abnormal expression pattern of miR‑135a coincided with highly expressed endogenous factors, including GATA binding protein (GATA)‑3 and interleukin (IL)‑4, and lowly expressed factors, including T‑box expressed in T cells (T‑bet) and interferon (IFN)‑γ. We hypothesized that miR‑135a may play an important role in immune regulation in AR mice. In the present study, AR was induced by OVA in the mice. Two groups of the AR mice were treated with a miR‑135a mimic and a mimic control, respectively. The serum and nasal mucosa were collected for analysis. Following miR‑135a application, the serum OVA‑sIgE concentration was significantly reduced. In the nasal mucosa, the expression levels of miR‑135a were higher, the mRNA and protein expression levels of GATA‑3 and IL‑4 were lower, and the expression levels of T‑bet and IFN‑γ were higher. The miR‑135a corrected the Th1/Th2 imbalance in the AR mice. Findings of this study may provide a basis for novel genetic treatments in addressing allergic diseases.

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