Tumor necrosis factor‑α promotes the expression of excitatory amino‑acid transporter 2 in astrocytes: Optimal concentration and incubation time

Ding,Yuemin; Zhang,Kena; Liu,Shuqin; Zhang,Qijun; Ma,Chiyuan; Bruce,Iain  C.; Zhang,Xiong

doi:10.3892/etm.2014.2024

Tumor necrosis factor‑α promotes the expression of excitatory amino‑acid transporter 2 in astrocytes: Optimal concentration and incubation time

Authors:
- Yuemin Ding
- Kena Zhang
- Shuqin Liu
- Qijun Zhang
- Chiyuan Ma
- Iain C. Bruce
- Xiong Zhang
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Affiliations: School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang 310015, P.R. China, College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Pharmacy, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Basic Medicine, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China
Published online on: October 15, 2014 https://doi.org/10.3892/etm.2014.2024
Pages: 1909-1913

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Abstract

The aim of the present study was to determine whether tumor necrosis factor (TNF)‑α regulates the expression levels of excitatory amino‑acid transporters (EAATs) in primary astrocytes and its roles in brain ischemia. Exogenous TNF‑α was administered to pure cultured astrocytes and the expression levels of EAAT1, EAAT2 and glial fibrillary acidic protein (GFAP) were evaluated. The results showed that TNF‑α at 10 ng/ml enhanced the expression of EAAT2 in a time‑dependent manner, while the expression levels of EAAT1 and GFAP did not change. To determine whether the elevation in the levels of the EAAT2 protein induced by TNF‑α had a beneficial effect on ischemic insult, TNF‑α was applied to in vitro models of cerebral ischemia; the treatment was observed to increase neuronal viability. The present results suggest that a relatively short‑term application of an optimal concentration of TNF‑α may protect neurons against ischemic injury by elevating the expression of EAAT2 in astrocytes.

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