Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells

  • Authors:
    • Liqun Xie
    • Zexing Duan
    • Caiju Liu
    • Yanmin Zheng
    • Jing Zhou
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  • Published online on: November 5, 2014     https://doi.org/10.3892/etm.2014.2052
  • Pages: 239-244
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Abstract

The aim of this study was to determine the expression of protease‑activated receptor 2 (PAR‑2) in the human pancreatic cancer cell line SW1990, and to evaluate its effect on cell proliferation and invasion. The expression of PAR‑2 protein and mRNA in SW1990 cells was determined by immunocytochemistry and reverse transcription polymerase chain reaction (PCR), respectively. MTT and cell invasion and migration assays, as well as semi‑quantitative PCR and zymography analysis, were additionally performed. PAR‑2 mRNA was significantly upregulated in the cells treated with trypsin or the PAR‑2 activating peptide Ser‑Leu‑Ile‑Gly‑Lys‑Val (SLIGKV) (P<0.01), but not in the Val‑Lys‑Gly‑Ile‑Leu‑Ser group (P>0.05). Trypsin and SLIGKV significantly promoted SW1990 cell proliferation in a dose‑ and time‑dependent manner (P<0.05). Compared with the control group, trypsin and SLIGKV significantly increased the mRNA expression (P<0.01) and gelatinolytic activity (P<0.01) of matrix metalloproteinase (MMP)‑2. In conclusion, PAR‑2 is expressed in SW1990 cells. PAR‑2 activation may promote the invasion and migration of human pancreatic cancer cells by increasing MMP‑2 expression.
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January-2015
Volume 9 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xie L, Duan Z, Liu C, Zheng Y and Zhou J: Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells. Exp Ther Med 9: 239-244, 2015.
APA
Xie, L., Duan, Z., Liu, C., Zheng, Y., & Zhou, J. (2015). Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells. Experimental and Therapeutic Medicine, 9, 239-244. https://doi.org/10.3892/etm.2014.2052
MLA
Xie, L., Duan, Z., Liu, C., Zheng, Y., Zhou, J."Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells". Experimental and Therapeutic Medicine 9.1 (2015): 239-244.
Chicago
Xie, L., Duan, Z., Liu, C., Zheng, Y., Zhou, J."Protease‑activated receptor 2 agonist increases cell proliferation and invasion of human pancreatic cancer cells". Experimental and Therapeutic Medicine 9, no. 1 (2015): 239-244. https://doi.org/10.3892/etm.2014.2052