Erythropoietin protects lipopolysaccharide-induced renal mesangial cells from autophagy

Bi,Lingyun; Hou,Ruanling; Yang,Dasheng; Li,Shujun; Zhao,Dean

doi:10.3892/etm.2014.2124

Erythropoietin protects lipopolysaccharide-induced renal mesangial cells from autophagy

Authors:
- Lingyun Bi
- Ruanling Hou
- Dasheng Yang
- Shujun Li
- Dean Zhao
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Affiliations: Department of Pediatrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Physiology Laboratory, Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China
Published online on: December 10, 2014 https://doi.org/10.3892/etm.2014.2124
Pages: 559-562

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Abstract

The aim of this study was to investigate the effects of erythropoietin (EPO) on the impairment of autophagy induced by lipopolysaccharide (LPS) in primary cultured rat glomerular mesangial cells (GMCs). Rat GMCs were isolated and cultured in normal glucose, high‑glucose, LPS or LPS + EPO medium. At 24 and 72 h of culture, the cells were examined for expression levels of the autophagy markers LC3 and p62/sequestosome‑1 (SQSTM1) using western blot analysis. At 24 h, no significant difference in the expression of LC3 and p62/SQSTM1 was observed among the groups; however, the cells exposed to high‑glucose medium for 72 h showed downregulated LC3 expression and upregulated p62/SQSTM1 expression. The cells exposed to LPS (10 ng/ml) for 72 h showed upregulated LC3 expression and upregulated p62/SQSTM1 expression. These changes were reversed in the LPS + EPO group at 72 h. In conclusion, EPO can inhibit LPS‑induced autophagy in rat GMCs.

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