Correlation between the -1562C/T polymorphism in the matrix metalloproteinase-9 gene and hemorrhagic transformation of ischemic stroke

Zhang,Xiaoman; Cao,Xinhui; Xu,Xiaoyu; Li,Aifan; Xu,Yuming

doi:10.3892/etm.2015.2186

Correlation between the -1562C/T polymorphism in the matrix metalloproteinase-9 gene and hemorrhagic transformation of ischemic stroke

Authors:
- Xiaoman Zhang
- Xinhui Cao
- Xiaoyu Xu
- Aifan Li
- Yuming Xu
View Affiliations

Affiliations: Department of Neurology, The First People's Hospital of Zhengzhou, Zhengzhou, Henan 450004, P.R. China, Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: January 16, 2015 https://doi.org/10.3892/etm.2015.2186
Pages: 1043-1047

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the correlation between the ‑1562C/T polymorphism in an intron of the matrix metalloproteinase‑9 (MMP‑9) gene and hemorrhagic transformation of ischemic stroke (IS). Using polymerase chain reaction‑restriction fragment length polymorphism, the ‑1562C/T polymorphisms in 222 patients with IS were detected. The patients were divided into hemorrhagic transformation (HT; 84 cases) and non‑hemorrhagic transformation (NHT) groups (138 cases) depending on the results from the susceptibility‑weighted magnetic resonance imaging, which was performed between one and two weeks following stroke onset. The allele frequencies were subsequently compared. Baseline data of the two groups were comparable. The HT group exhibited a significantly lower frequency of the CT+TT genotype compared with the NHT group (17.86 vs. 30.43%, P<0.05). In addition, the frequency of T allele was significantly lower in the HT group compared with the NHT group (8.93 vs. 15.94%, P<0.05). Therefore, the results indicated that the ‑1562C/T polymorphism in the MMP‑9 gene is correlated with hemorrhagic transformation of IS in the population studied. Furthermore, the T allele may be a protective factor for hemorrhagic transformation of IS in this population.

View Figures

View References

1	Barroso B: Decompressive craniectomy for stroke after intravenous thombolytic therapy. Int J Stroke. 9:E402014. View Article : Google Scholar
2	Yang J, Zheng M, Chen S, et al: Knowledge of thrombolytic therapy for acute ischemic stroke among community residents in western urban China. PLoS One. 9:e1078922014. View Article : Google Scholar : PubMed/NCBI
3	Gargiulo S, Sottero B, Gamba P, Chiarpotto E, Poli G and Leonarduzzi G: Plaque oxysterols induce unbalanced up-regulation of matrix metalloproteinase-9 in macrophagic cells through redox-sensitive signaling pathways: Implications regarding the vulnerability of atherosclerotic lesions. Free Radic Biol Med. 51:844–855. 2011. View Article : Google Scholar : PubMed/NCBI
4	Pan CL and Zheng LF: Relationship of lysophosphatidic acid and matrix metalloproteinase-9 with carotid atheromatous plaque stability in patients with cerebral infarction. Zhonghua Lao Nian Yi Xue Za Zhi. 29:115–118. 2010.(In Chinese).
5	Pöllänen PJ, Karhunen PJ, Mikkelsson J, et al: Coronary artery complicated lesion area is related to functional polymorphism of matrix metalloproteinase 9 gene: an autopsy study. Arterioscler Thromb Vasc Biol. 21:1446–1450. 2001. View Article : Google Scholar : PubMed/NCBI
6	Chinese Neuroscience, Chinese Academy of Neuroscience. Various types of cerebrovascular disease diagnostic criteria. Zhonghua Shen Jing Ke Za Zhi. 29:379–380. 1996.(In Chinese).
7	Ahmad NN, Cu-Unjieng AB and Donoso LA: Modification of standard proteinase K/phenol method for DNA isolation to improve yield and purity from frozen blood. J Med Genet. 32:129–130. 1995. View Article : Google Scholar : PubMed/NCBI
8	Cao XH, Liu M and Wu B: Relationship between plasma matrix metalloproteinase-9 and hemorrhagic transformation in ischemic stroke: a systematic review. Zhongguo Xun Zheng Yi Xue Za Zhi. 6:361–369. 2006.(In Chinese).
9	Hou H, Zhang G, Wang H, Gong H, Wang C and Zhang X: High matrix metalloproteinase-9 expression induces angiogenesis and basement membrane degradation in stroke-prone spontaneously hypertensive rats after cerebral infarction. Neural Regen Res. 9:1154–1162. 2014. View Article : Google Scholar : PubMed/NCBI
10	Sarrafzadeh A, Copin JC, Bengualid DJ, et al: Matrix metalloproteinase-9 concentration in the cerebral extracellular fluid of patients during the acute phase of aneurysmal subarachnoid hemorrhage. Neurol Res. 34:455–461. 2012. View Article : Google Scholar : PubMed/NCBI
11	Wang X and Shi LZ: Association of matrix metalloproteinase-9 C1562T polymorphism and coronary artery disease: a meta-analysis. J Zhejiang Univ Sci B. 15:256–263. 2014. View Article : Google Scholar : PubMed/NCBI
12	Schveigert D, Valuckas KP, Kovalcis V, Ulys A, Chvatovic G and Didziapetriene J: Significance of MMP-9 expression and MMP-9 polymorphism in prostate cancer. Tumori. 99:523–529. 2013.PubMed/NCBI
13	Xiao Z, Liu Q, Mao F, Wu J and Lei T: TNF-α-induced VEGF and MMP-9 expression promotes hemorrhagic transformation in pituitary adenomas. Int J Mol Sci. 12:4165–4179. 2011. View Article : Google Scholar
14	Montaner J, Molina CA, Monasterio J, et al: Matrix metalloproteinase-9 pretreatment level predicts intracranial hemorrhagic complications after thrombolysis in human stroke. Circulation. 107:598–603. 2003. View Article : Google Scholar : PubMed/NCBI
15	Jiang XB, Wang JS, Liu DH, Yuan WS and Shi ZS: Overexpression of matrix metalloproteinase-9 is correlated with carotid intraplaque hemorrhage in a swine model. J Neurointerv Surg. 5:473–477. 2013. View Article : Google Scholar
16	Goracy J, Goracy I, Brykczyński M, Peregud-Pogorzelska M, Naruszewicz M and Ciechanowicz A: The C(−1562)T polymorphism in the promoter of the matrix metalloproteinase-9 (MMP-9) gene and coronary atherosclerosis. Pol Arch Med Wewn. 110:1275–1281. 2003.(In Polish).
17	Szczudlik P and Borratyńska A: Association between the −1562 C/T MMP-9 polymorphism and cerebrovascular disease in a Polish population. Neurol Neurochir Pol. 44:350–357. 2010.PubMed/NCBI
18	Fernández-Cadenas I, Del Río-Espínola A, Carrera C, et al: Role of the MMP9 gene in hemorrhagic transformations after tissue-type plasminogen activator treatment in stroke patients. Stroke. 43:1398–1400. 2012. View Article : Google Scholar : PubMed/NCBI
19	Tang LJ, Chen XF, Zhu M, Shen WF and Jiang JJ: Study of relations between matrix metalloproteinase-9 polymorphism (C−1562T) and acute coronary syndrome in Han population of China. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 22:313–316. 2005.(In Chinese). PubMed/NCBI
20	Song WH, Dang AM, Zhu JM, Lü NQ, Liu GZ and Hui RT: MMP-9 gene −1562C/T polymorphism in aortic dissection in Chinese hypertensive patients. Zhonghua Nei Ke Za Zhi. 45:376–378. 2006.(In Chinese). PubMed/NCBI
21	Duellman T, Warren CL, Peissig P, Wynn M and Yang J: Matrix metalloproteinase-9 genotype as a potential genetic marker for abdominal aortic aneurysm. Circ Cardiovasc Genet. 5:529–537. 2012. View Article : Google Scholar : PubMed/NCBI
22	Xing LL, Wang ZN, Jiang L, et al: Matrix metalloproteinase-9−1562C>T polymorphism may increase the risk of lymphatic metastasis of colorectal cancer. World J Gastroenterol. 13:4626–4629. 2007.PubMed/NCBI
23	Xin DD, Chen ZB, Liu XP, et al: Association of functional −1562C/T polymorphism in matrix metalloproteinase-9 (MMP-9) with risk of severe encephalitis in enterovirus 71 infected children. Xian Dai Sheng Wu Yi Xue Jin Zhan. 15:2870–2874. 2013.(In Chinese).