Effect of picroside II on apoptosis induced by renal ischemia/reperfusion injury in rats

Wang,Lei; Liu,Xiuheng; Chen,Hui; Chen,Zhiyuan; Weng,Xiaodong; Qiu,Tao; Liu,Lin

doi:10.3892/etm.2015.2192

Effect of picroside II on apoptosis induced by renal ischemia/reperfusion injury in rats

Authors:
- Lei Wang
- Xiuheng Liu
- Hui Chen
- Zhiyuan Chen
- Xiaodong Weng
- Tao Qiu
- Lin Liu
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Affiliations: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: January 20, 2015 https://doi.org/10.3892/etm.2015.2192
Pages: 817-822

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Abstract

Renal ischemia and reperfusion (I/R) injury, which commonly occurs in kidney transplantation, is the leading cause of acute kidney injury. Picroside II possesses a wide range of pharmacological effects, including anti‑apoptosis effects. In the present study, the ability of picroside II to attenuate apoptosis in a rat model of renal I/R injury was investigated. Sprague‑Dawley rats were subjected to 45 min of ischemia followed by 24 h of reperfusion. Prior to reperfusion, the rats were treated with picroside II or an equal volume of phosphate‑buffered saline. It was observed that renal function was significantly improved by the treatment with picroside II. Morphological analysis indicated that picroside II markedly reduced tissue damage and the expression of cleaved caspase‑3. Reverse transcription‑quantitative polymerase chain reaction and western blotting revealed that the expression levels of Bax and poly(ADP‑ribose) polymerase‑1 (PARP‑1) were upregulated in the I/R group, whereas those of Bcl‑2 were downregulated. However, the treatment with picroside II inhibited these changes induced by renal I/R injury. In conclusion, picroside II has potent anti‑apoptotic activity against renal I/R injury.

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