Preconditioning with PEP-1-SOD1 fusion protein attenuates ischemia/reperfusion-induced ventricular arrhythmia in isolated rat hearts

Ke,Zunping; Gao,Aimei; Xu,Peng; Wang,Jianing; Ji,Lijuan; Yang,Jianye

doi:10.3892/etm.2015.2440

Preconditioning with PEP-1-SOD1 fusion protein attenuates ischemia/reperfusion-induced ventricular arrhythmia in isolated rat hearts

Authors:
- Zunping Ke
- Aimei Gao
- Peng Xu
- Jianing Wang
- Lijuan Ji
- Jianye Yang
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Affiliations: Department of Cardiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200040, P.R. China, Department of Pharmacy, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200040, P.R. China, Institute of Clinical Medicine and Department of Cardiology, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Rehabilitation, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223001, P.R. China
Published online on: April 22, 2015 https://doi.org/10.3892/etm.2015.2440
Pages: 352-356

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Abstract

PEP 1-Cu/Zn superoxide dismutase (PEP-1-SOD1) fusion protein preconditioning has been reported to protect the myocardium from ischemia/reperfusion (I/R)‑induced injury by decreasing the infarct size, reducing levels of cardiomyocyte apoptosis and reducing the release of myocardial‑specific biomarkers. The aim of the present study was to examine the effects of PEP‑1‑SOD1 pretreatment on I/R‑induced ventricular arrhythmias in Langendorff‑perfused rat hearts. The isolated rat hearts were pretreated with PEP‑1‑SOD1 prior to I/R, and the I/R‑induced hemodynamic parameters, infarct size and ventricular arrhythmias were then assessed. Compared with the unprotected hearts, PEP‑1‑SOD1 preconditioning significantly improved the hemodynamic parameters, decreased the cardiac lactate dehydrogenase and creatine kinase‑MB (CK‑MB) levels, reduced the infarct size and attenuated the ventricular arrhythmia. Further investigation showed that PEP‑1‑SOD1 preconditioning reduced both the incidence and duration of ventricular tachycardia/ventricular fibrillation. In addition, the intracellular reactive oxygen species (ROS) levels were decreased. The results of the present study suggest that PEP‑1‑SOD1 preconditioning can protect the heart against I/R injury and attenuate I/R-induced arrhythmia by downregulating the generation of ROS.

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