Ischemic postconditioning attenuates inflammation in rats following renal ischemia and reperfusion injury

Chen,Hui; Wang,Lei; Xing,Bian‑Zhi; Liu,Xiu‑Heng; Chen,Zhi‑Yuan; Weng,Xiao‑Dong; Qiu,Tao; Liu,Lin

doi:10.3892/etm.2015.2514

Ischemic postconditioning attenuates inflammation in rats following renal ischemia and reperfusion injury

Authors:
- Hui Chen
- Lei Wang
- Bian‑Zhi Xing
- Xiu‑Heng Liu
- Zhi‑Yuan Chen
- Xiao‑Dong Weng
- Tao Qiu
- Lin Liu
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Affiliations: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Radiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: May 22, 2015 https://doi.org/10.3892/etm.2015.2514
Pages: 513-518

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Abstract

Ischemic postconditioning (IPoC) involves a series of brief rapid intermittent ischemic episodes applied at the onset of reperfusion in the previously ischemic tissue or organ. Previous studies have demonstrated that IPoC attenuates tissue damage induced by ischemia and reperfusion (I/R) injury. The aim of the present study was to investigate whether IPoC has a beneficial effect on inflammation in a rat model of renal I/R injury. Wistar rats were subjected to 45 min of ischemia followed by 24, 72 or 120 h of reperfusion (I/R group). In the IPoC group, rats subjected to I/R were treated with six cycles of 10 sec reperfusion followed by a 10‑sec ischemic episode. Blood samples were collected for the determination of blood urea nitrogen (BUN) and creatinine (Cr) levels. Furthermore, histological examination and immunohistochemical staining for the localization of nuclear factor‑κB (NF‑κB) were performed. In addition, quantitative polymerase chain reaction (qPCR) analysis was used to determine the expression levels of intercellular adhesion molecule‑1 (ICAM‑1), interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α), while western blot analysis was used to detect the protein expression levels of NF‑κB. The results indicated that the BUN and Cr levels increased significantly in the I/R group, while the IPoC rats showed evidently reduced renal damage. Immunohistochemical analysis revealed that the expression levels of NF‑κB were decreased by IPoC. In addition, the qPCR results revealed that IPoC significantly inhibited the increased mRNA expression levels of ICAM‑1, IL‑6 and TNF‑α, induced by I/R injury. Western blot analysis indicated that the expression levels of NF‑κB were upregulated in the I/R group, while IPoC was shown to inhibit the expression. In conclusion, IPoC was demonstrated to exhibit potent anti‑inflammatory properties against renal I/R injury.

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1	Kam Tao Li P, Burdmann EA and Mehta RLWorld Kidney Day Steering Committee 2013: Acute kidney injury: Global health alert. J Nephropathol. 2:90–97. 2013. View Article : Google Scholar : PubMed/NCBI
2	Yun Y, Duan WG, Chen P, Wu HX, Shen ZQ, Qian ZY and Wang DH: Ischemic postconditioning modified renal oxidative stress and lipid peroxidation caused by ischemic reperfusion injury in rats. Transplant Proc. 41:3597–3602. 2009. View Article : Google Scholar : PubMed/NCBI
3	Barri YM, Sanchez EQ, Jennings LW, Melton LB, Hays S, Levy MF and Klintmalm GB: Acute kidney injury following liver transplantation: definition and outcome. Liver Transpl. 15:475–483. 2009. View Article : Google Scholar : PubMed/NCBI
4	Ma ZF, Chen W, Cao CC and Chen X: Ischemic preconditioning attenuates brain injury induced by ischemia/reperfusion during moderate hypothermia low-flow procedures. Int J Neurosci. Jan 24–2014.(Epub ahead of print). View Article : Google Scholar
5	Ma J, Qiao Z and Xu B: Effects of ischemic preconditioning on myocardium Caspase-3, SOCS-1, SOCS-3, TNF-α and IL-6 mRNA expression levels in myocardium IR rats. Mol Biol Rep. 40:5741–5748. 2013. View Article : Google Scholar : PubMed/NCBI
6	Zhao ZQ, Corvera JS, Halkos ME, Kerendi F, Wang NP, Guyton RA and Vinten-Johansen J: Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. Am J Physiol Heart Circ Physiol. 285:H579–H588. 2003. View Article : Google Scholar : PubMed/NCBI
7	Kong Y, Rogers MR and Qin X: Effective neuroprotection by ischemic postconditioning is associated with a decreased expression of RGMa and inflammation mediators in ischemic rats. Neurochem Res. 38:815–825. 2013. View Article : Google Scholar : PubMed/NCBI
8	Wu H, Lei S, Yuan J, Liu X, Zhang D, Gu X, Zhang L and Xia Z: Ischemic postconditioning downregulates Egr-1 expression and attenuates postischemic pulmonary inflammatory cytokine release and tissue injury in rats. J Surg Res. 181:204–212. 2013. View Article : Google Scholar : PubMed/NCBI
9	Chen H, Xing B, Liu X, Zhan B, Zhou J, Zhu H and Chen Z: Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. Eur J Pharmacol. 581:306–314. 2008. View Article : Google Scholar : PubMed/NCBI
10	Chen H, Xing B, Liu X, Zhan B, Zhou J, Zhu H and Chen Z: Ischemic postconditioning inhibits apoptosis after renal ischemia/reperfusion injury in rat. Transpl Int. 21:364–371. 2008. View Article : Google Scholar : PubMed/NCBI
11	Jablonski P, Howden BO, Rae DA, Birrell CS, Marshall VC and Tange J: An experimental model for assessment of renal recovery from warm ischemia. Transplantation. 35:198–204. 1983. View Article : Google Scholar : PubMed/NCBI
12	Granfeldt A, Lefer DJ and Vinten-Johansen J: Protective ischaemia in patients: preconditioning and postconditioning. Cardiovasc Res. 83:234–246. 2009. View Article : Google Scholar : PubMed/NCBI
13	Venugopal V, Ludman A, Yellon DM and Hausenloy DJ: ‘Conditioning’ the heart during surgery. Eur J Cardiothorac Surg. 35:977–987. 2009. View Article : Google Scholar : PubMed/NCBI
14	Zhao ZQ: Postconditioning in reperfusion injury: a status report. Cardiovasc Drugs Ther. 24:265–279. 2010. View Article : Google Scholar : PubMed/NCBI
15	Wang JQ, Chen X, Zhang C, Tao L, Zhang ZH, Liu XQ, Xu YB, Wang H, Li J and Xu DX: Phenylbutyric acid protects against carbon tetrachloride-induced hepatic fibrogenesis in mice. Toxicol Appl Pharmacol. 266:307–316. 2013. View Article : Google Scholar : PubMed/NCBI
16	Wang Y, Ge P and Zhu Y: TLR2 and TLR4 in the brain injury caused by cerebral ischemia and reperfusion. Mediators Inflamm. 2013:1246142013. View Article : Google Scholar : PubMed/NCBI
17	Carden DL and Granger DN: Pathophysiology of ischaemia-reperfusion injury. J Pathol. 190:255–266. 2000. View Article : Google Scholar : PubMed/NCBI
18	Ma JQ, Liu CM, Qin ZH, Jiang JH and Sun YZ: Ganoderma applanatum terpenes protect mouse liver against benzo(α)pyren-induced oxidative stress and inflammation. Environ Toxicol Pharmacol. 31:460–468. 2011. View Article : Google Scholar : PubMed/NCBI
19	Li YW, Zhang Y, Zhang L, Li X, Yu JB, Zhang HT, Tan BB, Jiang LH, Wang YX, Liang Y, et al: Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κB p65 signal pathway. Gene. 542:46–51. 2014. View Article : Google Scholar : PubMed/NCBI
20	Wong ET and Tergaonkar V: Roles of NF-kappaB in health and disease: mechanisms and therapeutic potential. Clin Sci (Lond). 116:451–465. 2009. View Article : Google Scholar : PubMed/NCBI
21	Zhang P, Liu X, Zhu Y, Chen S, Zhou D and Wang Y: Honokiol inhibits the inflammatory reaction during cerebral ischemia reperfusion by suppressing NF-κB activation and cytokine production of glial cells. Neurosci Lett. 534:123–127. 2013. View Article : Google Scholar : PubMed/NCBI
22	van den Akker EK, Hesselink DA, Manintveld OC, et al: Ischemic postconditioning in human DCD kidney transplantation is feasible and appears safe. Transpl Int. 27:226–234. 2014. View Article : Google Scholar : PubMed/NCBI
23	Weng X, Shen H, Kuang Y, Liu X, Chen Z, Zhu H, Jiang B, Zhu G and Chen H: Ischemic postconditioning inhibits the renal fibrosis induced by ischemia-reperfusion injury in rats. Urology. 80:484.e1–484.e7. 2012. View Article : Google Scholar