Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head

Bai,Rui; Liu,Wanlin; Zhao,Aiqing; Zhao,Zhengqun; Jiang,Dianming

doi:10.3892/etm.2015.2521

Nitric oxide content and apoptosis rate in steroid-induced avascular necrosis of the femoral head

Authors:
- Rui Bai
- Wanlin Liu
- Aiqing Zhao
- Zhengqun Zhao
- Dianming Jiang
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Affiliations: Graduate School of Chongqing Medical University, Chongqing, P.R. China, Department of Pediatric Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, P.R. China, Department of Computed Tomography, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia, P.R. China, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China
Published online on: May 26, 2015 https://doi.org/10.3892/etm.2015.2521
Pages: 591-597

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Abstract

The aim of the present study was to explore the effect on nitric oxide (NO) content and osteocyte apoptosis of steroid‑induced avascular necrosis of the femoral head (SANFH) in an animal model of SANFH. A total of 40 Japanese white rabbits, 5 months of age and weighing 2.5±0.5 kg, were randomly divided into groups A (hormone + endotoxin group), B (endotoxin + normal saline group), C (normal saline + hormone group) and D (control group). Following the establishment of the model, a blood sample was taken from the heart of each animal and centrifuged; the levels of NO in the serum were detected. The bilateral femoral heads were conventionally dissected, fixed, decalcified and stained with hematoxylin and eosin. Subsequently, the empty bone lacunae were counted under an optical microscope. Changes in osteocyte morphology were observed using electron microscopy and osteocyte apoptosis was detected with a terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The percentage of empty bone lacunae in group A was significantly higher compared with that in groups B, C and D (P<0.01); however, there was no significant difference in percentage among groups B, C and D. The NO content in group A was significantly higher compared with that in groups B, C and D (P<0.01); however, there was no significant difference in NO content among groups B, C and D. The osteocyte apoptosis index in group A was significantly higher compared with that in the other groups (P<0.01); there was no significant difference among groups B, C and D. NO content was positively correlated with osteocyte apoptosis index (r=0.707). Thus, the present study found that NO content and the osteocyte apoptosis index were increased in SANFH, and that they play an important role in SANFH. The content of NO was positively correlated with the osteocyte apoptosis index, indicating that NO induces apoptosis.

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