Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro

Tang,Xiao‑Bo; Dong,Pei‑Long; Wang,Jian; Zhou,Hai‑Yang; Zhang,Hai‑Xiang; Wang,Shan‑Zheng

doi:10.3892/etm.2015.2528

Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro

Authors:
- Xiao‑Bo Tang
- Pei‑Long Dong
- Jian Wang
- Hai‑Yang Zhou
- Hai‑Xiang Zhang
- Shan‑Zheng Wang
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Affiliations: Department of Orthopedics, Jianhu Hospital, Medical School of Nantong University, Jianhu, Jiangsu 224700, P.R. China, Department of Orthopedics, Nanjing Integrative Medicine Hospital, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210014, P.R. China, Department of Orthopedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China
Published online on: May 28, 2015 https://doi.org/10.3892/etm.2015.2528
Pages: 477-483
Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study aimed to isolate rabbit adipose-derived stem cells (ADSCs) and explore the potential of platelet‑rich plasma (PRP) in the chondrogenic differentiation of ADSCs, thereby potentially providing a new approach for the repair and regeneration of cartilage injury. Rabbit ADSCs were isolated and characterized by induction towards adipogenic, osteogenic and chondrogenic lineages in vitro. The isolated ADSCs were also cultured with or without 10% PRP. Immunofluorescence staining, toluidine blue staining and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were used to detect type II collagen (Col II) and aggrecan (AGC) expression. Col II immunofluorescence staining and toluidine blue staining indicated that following induction by autologous PRP, ADSCs manifested Col II and AGC expression. The expression of Col II and AGC mRNA was significantly upregulated in the PRP‑treated cells when compared with that in control cells. Autologous PRP produced by laboratory centrifugation was able to promote the chondrogenic differentiation of rabbit ADSCs in vitro.

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1	Lim JY, Loiselle AE, Lee JS, et al: Optimizing the osteogenic potential of adult stem cells for skeletal regeneration. J Orthop Res. 29:1627–1633. 2011. View Article : Google Scholar : PubMed/NCBI
2	Gimble JM and Guilak F: Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 5:362–369. 2003. View Article : Google Scholar : PubMed/NCBI
3	Marx RE: Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent. 10:225–228. 2001. View Article : Google Scholar : PubMed/NCBI
4	Knighton DR, Hunt TK, Thakral KK and Goodson WH III: Role of platelets and fibrin in the healing sequence: an in vivo study of angiogenesis and collagen synthesis. Ann Surg. 196:379–388. 1982. View Article : Google Scholar : PubMed/NCBI
5	Brass L: Understanding and evaluating platelet function. Hematology Am Soc Hematol Educ Program. 2010:387–396. 2010. View Article : Google Scholar : PubMed/NCBI
6	Zhang N, Wu YP, Qian SJ, et al: Research progress in the mechanism of effect of PRP in bone deficiency healing. ScientificWorld Journal. 2013:1345822013. View Article : Google Scholar : PubMed/NCBI
7	Lenza M, Ferraz Sde B, Viola DC, et al: Platelet-rich plasma for long bone healing. Einstein (Sao Paulo). 11:122–127. 2013. View Article : Google Scholar : PubMed/NCBI
8	Lubkowska A, Dolegowska B and Banfi G: Growth factor content in PRP and their applicability in medicine. J Biol Regul Homeost Agents. 26 (2 Suppl 1):3S–22S. 2012.PubMed/NCBI
9	Lee HR, Park KM, Joung YK, et al: Platelet-rich plasma loaded in situ-formed hydrogel enhances hyaline cartilage regeneration by CB1 upregulation. J Biomed Mater Res A. 100:3099–3107. 2012. View Article : Google Scholar : PubMed/NCBI
10	Wearing SC, Hennig EM, Byrne NM, et al: Musculoskeletal disorders associated with obesity: a biomechanical perspective. Obes Rev. 7:239–250. 2006. View Article : Google Scholar : PubMed/NCBI
11	Johnstone B, Alini M, Cucchiarini M, et al: Tissue engineering for articular cartilage repair - the state of the art. Eur Cell Mater. 25:248–267. 2013.PubMed/NCBI
12	Ye K, Felimban R, Moulton SE, et al: Bioengineering of articular cartilage: past, present and future. Regen Med. 8:333–349. 2013. View Article : Google Scholar : PubMed/NCBI
13	Rui YF, Lui PP, Lee YW and Chan KM: Higher BMP receptor expression and BMP-2-induced osteogenic differentiation in tendon-derived stem cells compared with bone-marrow-derived mesenchymal stem cells. Int Orthop. 6:1099–1107. 2012. View Article : Google Scholar
14	Nagae M, Ikeda T, Mikami Y, et al: Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Eng. 13:147–158. 2007. View Article : Google Scholar : PubMed/NCBI
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2007. View Article : Google Scholar
16	Kock L, van Donkelaar CC and Ito K: Tissue engineering of functional articular cartilage: the current status. Cell Tissue Res. 347:613–627. 2012. View Article : Google Scholar : PubMed/NCBI
17	Johnstone B and Yoo J: Mesenchymal cell transfer for articular cartilage repair. Expert Opin Biol Ther. 1:915–921. 2001. View Article : Google Scholar : PubMed/NCBI
18	Tollervey JR and Lunyak VV: Adult stem cells: simply a tool for regenerative medicine or an additional piece in the puzzle of human aging? Cell Cycle. 10:4173–4176. 2011. View Article : Google Scholar : PubMed/NCBI
19	Janicki P and Schmidmaier G: What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells. Injury. 42 (Suppl 2):77–81. 2011. View Article : Google Scholar
20	Lui PP, Rui YF, Ni M and Chan KM: Tenogenic differentiation of stem cells for tendon repair - what is the current evidence? J Tissue Eng Regen Med. 5:e144–e163. 2011. View Article : Google Scholar : PubMed/NCBI
21	Huang S, Tam V, Cheung KM, et al: Stem cell-based approaches for intervertebral disc regeneration. Curr Stem Cell Res Ther. 6:317–326. 2011. View Article : Google Scholar : PubMed/NCBI
22	Lubis AM and Lubis VK: Adult bone marrow stem cells in cartilage therapy. Acta Med Indones. 44:62–68. 2012.PubMed/NCBI
23	Zuk PA, Zhu M, Mizuno H, et al: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI
24	Casteilla L, Planat-Benard V, Bourin P, et al: Use of adipose tissue in regenerative medicine. Transfus Clin Biol. 18:124–128. 2011.(In French). View Article : Google Scholar : PubMed/NCBI
25	Markarian CF, Frey GZ, Silveira MD, et al: Isolation of adipose-derived stem cells: a comparison among different methods. Biotechnol Lett. 36:693–702. 2014. View Article : Google Scholar : PubMed/NCBI
26	Griffin M, Hindocha S and Khan WS: Chondrogenic differentiation of adult MSCs. Curr Stem Cell Res Ther. 7:260–265. 2012. View Article : Google Scholar : PubMed/NCBI
27	Freyria AM, Courtes S and Mallein-Gerin F: Differentiation of adult human mesenchymal stem cells: chondrogenic effect of BMP-2. Pathol Biol (Paris). 56:326–333. 2008.(In French). View Article : Google Scholar : PubMed/NCBI
28	Arnott JA, Lambi AG, Mundy C, et al: The role of connective tissue growth factor (CTGF/CCN2) in skeletogenesis. Crit Rev Eukaryot Gene Expr. 21:43–69. 2011. View Article : Google Scholar : PubMed/NCBI
29	Ellman MB, An HS, Muddasani P and Im HJ: Biological impact of the fibroblast growth factor family on articular cartilage and intervertebral disc homeostasis. Gene. 420:82–89. 2008. View Article : Google Scholar : PubMed/NCBI
30	Stewart AA, Byron CR, Pondenis H and Stewart MC: Effect of fibroblast growth factor-2 on equine mesenchymal stem cell monolayer expansion and chondrogenesis. Am J Vet Res. 68:941–945. 2007. View Article : Google Scholar : PubMed/NCBI
31	Van Osch GJ, Van Der Veen SW, Burger EH and Verwoerd-Verhoef HL: Chondrogenic potential of in vitro multiplied rabbit perichondrium cells cultured in alginate beads in defined medium. Tissue Eng. 6:321–330. 2000. View Article : Google Scholar : PubMed/NCBI
32	Barry F, Boynton RE, Liu B and Murphy JM: Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components. Exp Cell Res. 268:189–200. 2001. View Article : Google Scholar : PubMed/NCBI
33	Mardani M, Kabiri A, Esfandiari E, et al: The effect of platelet rich plasma on chondrogenic differentiation of human adipose derived stem cells in Transwell culture. Iran J Basic Med Sci. 16:1163–1169. 2013.PubMed/NCBI
34	Smyth NA, Murawski CD, Fortier LA, et al: Platelet-rich plasma in the pathologic processes of cartilage: review of basic science evidence. Arthroscopy. 29:1399–1409. 2013. View Article : Google Scholar : PubMed/NCBI
35	Anitua E, Andia I, Ardanza B, et al: Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 91:4–15. 2004.PubMed/NCBI
36	Krüger JP, Freymannx U, Vetterlein S, et al: Bioactive factors in platelet-rich plasma obtained by apheresis. Transfus Med Hemother. 40:432–440. 2013. View Article : Google Scholar : PubMed/NCBI