Silibinin attenuates methotrexate-induced pulmonary injury by targeting oxidative stress

Kalemci,Serdar; Topal,Yasar; Celik,Serkan  Yasar; Yilmaz,Nigar; Beydilli,Halil; Kosar,Mehmet  Ilkay; Dirican,Nigar; Altuntas,Irfan

doi:10.3892/etm.2015.2542

Silibinin attenuates methotrexate-induced pulmonary injury by targeting oxidative stress

Authors:
- Serdar Kalemci
- Yasar Topal
- Serkan Yasar Celik
- Nigar Yilmaz
- Halil Beydilli
- Mehmet Ilkay Kosar
- Nigar Dirican
- Irfan Altuntas
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Affiliations: Department of Chest Disease, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Pediatrics, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Pathology, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Biochemistry, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Emergency Medicine, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Anatomy, Medical Faculty, Mugla Sıtkı Kocman University, Mugla 48000, Turkey, Department of Chest Disease, Medical Faculty, Suleyman Demirel University, Isparta 32000, Turkey
Published online on: June 3, 2015 https://doi.org/10.3892/etm.2015.2542
Pages: 503-507
Copyright: © Kalemci et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to assess the protective effect of silibinin against methotrexate (MTX)-induced pulmonary toxicity. Rats were divided into four groups (MTX, MTX + silibinin, silibinin and control. MTX was injected intraperitoneally (i.p) into female Wistar rats (10 mg/kg/day for 3 days), which resulted in significant increases in the serum levels of alanine aminotransferase, aspartate aminotransferase and oxidant enzymes, including nitric oxide and myeloperoxidase. Furthermore, significant reductions were detected in the serum activity levels of the antioxidative enzymes, glutathione peroxidase and superoxide dismutase, when compared with the control group. However, administration of silibinin (100 mg/kg/day for 10 days, i.p.) was shown to ameliorate the MTX‑induced pulmonary toxicity, as indicated by the normalization of the oxidative stress parameters. Furthermore, silibinin treatment was demonstrated to reduce the histopathological changes associated with MTX. In conclusion, silibinin exhibited protective effects against MTX-induced pulmonary toxicity, which may be attributed to its antioxidant activity.

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