Effects of IgG anti-GM1 monoclonal antibodies on neuromuscular transmission and calcium channel binding in rat neuromuscular junctions

  • Authors:
    • Sayako Hotta
    • Yoshihiko Nakatani
    • Toshie Kambe
    • Kenji Abe
    • Yutaka Masuda
    • Iku Utsumomiya
    • Kyoji Taguchi
  • View Affiliations

  • Published online on: June 12, 2015     https://doi.org/10.3892/etm.2015.2575
  • Pages: 535-540
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Abstract

Guillain-Barré syndrome is a type of acute inflammatory neuropathy that causes ataxia and is associated with the IgG anti‑GM1 antibody. However, the pathogenic role of the IgG anti‑GM1 antibody and calcium channels in neuromuscular junctions (NMJs) remains unclear. Thus, the aim of the present study was to investigate the effects of the IgG anti‑GM1 monoclonal antibody (mAb) on spontaneous muscle action potentials (SMAPs), and the effects of calcium channel blockers, in a rat spinal cord‑muscle co‑culture system. In addition, the binding of IgG anti‑GM1 mAb to calcium channels was investigated in the rat hemidiaphragm. The frequency of SMAPs in the innervated muscle cells was acutely inhibited by the IgG anti‑GM1 mAb; however, this effect was blocked by the N‑type calcium channel blocker, ω‑conotoxin GVIA (30 nM). Furthermore, the P/Q‑type calcium channel blocker, ω‑agatoxin IVA (10 nM), was found to partially block the IgG anti‑GM1 mAb‑induced inhibitory effect in the spinal cord‑muscle co‑culture system. Immunohistochemical analysis of the rat hemidiaphragm indicated that IgG anti‑GM1 mAb binding overlapped with anti‑Cav2.2 (α1B) antibody binding in the nerve terminal. In addition, IgG anti‑GM1 mAb binding partially overlapped with anti‑Cav2.1 (α1A) antibody binding. Thus, the results demonstrated that the IgG anti‑GM1 mAb binds to calcium channels in the nerve terminals of NMJs. Therefore, the inhibitory effect of IgG anti‑GM1 mAb on SMAPs may involve N‑type and P/Q-type calcium channels in motor nerve terminals at the NMJ.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hotta S, Nakatani Y, Kambe T, Abe K, Masuda Y, Utsumomiya I and Taguchi K: Effects of IgG anti-GM1 monoclonal antibodies on neuromuscular transmission and calcium channel binding in rat neuromuscular junctions. Exp Ther Med 10: 535-540, 2015.
APA
Hotta, S., Nakatani, Y., Kambe, T., Abe, K., Masuda, Y., Utsumomiya, I., & Taguchi, K. (2015). Effects of IgG anti-GM1 monoclonal antibodies on neuromuscular transmission and calcium channel binding in rat neuromuscular junctions. Experimental and Therapeutic Medicine, 10, 535-540. https://doi.org/10.3892/etm.2015.2575
MLA
Hotta, S., Nakatani, Y., Kambe, T., Abe, K., Masuda, Y., Utsumomiya, I., Taguchi, K."Effects of IgG anti-GM1 monoclonal antibodies on neuromuscular transmission and calcium channel binding in rat neuromuscular junctions". Experimental and Therapeutic Medicine 10.2 (2015): 535-540.
Chicago
Hotta, S., Nakatani, Y., Kambe, T., Abe, K., Masuda, Y., Utsumomiya, I., Taguchi, K."Effects of IgG anti-GM1 monoclonal antibodies on neuromuscular transmission and calcium channel binding in rat neuromuscular junctions". Experimental and Therapeutic Medicine 10, no. 2 (2015): 535-540. https://doi.org/10.3892/etm.2015.2575