microRNA‑195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription‑3 signaling Retraction in /10.3892/etm.2023.12366

Zhao,Cheng; Qi,Lin; Chen,Minfeng; Liu,Longfei; Yan,Weiqian; Tong,Shiyu; Zu,Xiongbing

doi:10.3892/etm.2015.2633

microRNA‑195 inhibits cell proliferation in bladder cancer via inhibition of cell division control protein 42 homolog/signal transducer and activator of transcription‑3 signaling

Retraction in: /10.3892/etm.2023.12366

Authors:
- Cheng Zhao
- Lin Qi
- Minfeng Chen
- Longfei Liu
- Weiqian Yan
- Shiyu Tong
- Xiongbing Zu
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Affiliations: Department of Urology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Neurology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
Published online on: July 9, 2015 https://doi.org/10.3892/etm.2015.2633
Pages: 1103-1108

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Abstract

microRNA (miR)‑195 acts as a suppressor in multiple types of malignant tumors, including bladder cancer; however, the detailed function of miR‑195 in bladder cancer remains largely unknown. The aim of the present study was to investigate the role of miR‑195 in the regulation of bladder cancer cell proliferation and to determine whether cell division control protein 42 homolog (Cdc42)/signal transducer and activator of transcription‑3 (STAT3) signaling acts as a downstream effector of miR‑195 in bladder cancer cells. Reverse transcription‑quantitative polymerase chain reaction was used to determine the expression levels of miR‑195 in bladder cancer tissues and normal adjacent tissue. The results revealed that the expression of miR‑195 was significantly downregulated in bladder cancer tissues compared with that in the normal adjacent tissues. A luciferase reporter assay was then conducted, which identified Cdc42 as a direct target of miR‑195, and the expression of Cdc42 was significantly upregulated in bladder cancer tissues, as determined by western blotting. Furthermore, miR‑195 negatively regulated the protein expression of Cdc42 in bladder cancer cells. An MTT assay was also conducted to determine the rate of cell proliferation. Upregulation of miR‑195 or the inhibition of Cdc42 could inhibit bladder cancer cell proliferation, possibly through activation of STAT3 signaling. In addition, restoration of Cdc42 could reverse the inhibitory effect of miR‑195 upregulation on bladder cancer cell proliferation. In conclusion, the results of the present study suggest that miR‑195 plays an inhibitory role in the regulation of bladder cancer cell proliferation by directly targeting Cdc42/STAT3 signaling.

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