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Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress

  • Authors:
    • Shu‑Li Wang
    • De‑Shan Liu
    • Er‑Shun Liang
    • Yan‑Hua Gao
    • Ying Cui
    • Yu‑Zhao Liu
    • Wei Gao

  • View Affiliations

  • Published online on: August 24, 2015     https://doi.org/10.3892/etm.2015.2708
  • Pages: 1394-1400

  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study was designed to explore the protective effect of allicin on aortic endothelial cell injury induced by high glucose/hypoxia and to investigate the corresponding mechanisms. The primary‑cultured murine aortic endothelial cells were subcultured. The third passage of cells was adopted and randomly divided into five groups: The normal group (NG), the mannitol group (MG), the high‑glucose/hypoxia group (HG), the allicin group (AG) and the protein kinase C (PKC) inhibitor group (GG). The general morphology was observed under an inverted phase‑contrast microscope and cell viability was assessed using the MTT assay. Intracellular reactive oxygen species (ROS) levels in the endothelial cells were quantified using dihydroethidium staining. The levels of 8‑hydroxydeoxyguanosine (8‑OHdG), nuclear factor‑κB (NF‑κB), NADPH oxidase 4 (Nox4) and hypoxia‑inducible factor‑1α (HIF‑1α) and the activity of PKC were measured using ELISA. A quantitative polymerase chain reaction (qPCR) was adopted to evaluate the mRNA expression of Nox4, HIF‑1α and NF‑κB. The altered cell morphology observed in HG was notably ameliorated in the AG and GG. The protein levels of 8‑OHdG, NF‑κB, Nox4, HIF‑1α and PKC in the HG were higher than those in the other groups. Furthermore, the cell viability in the AG was significantly increased and the protein levels of 8‑OHdG, NF‑κB, Nox4, HIF‑1α and PKC were significantly decreased compared with those in the HG. The ROS production was found to be increased in the HG cells, while there was a significant decrease in the AG cells. These data indicate that allicin exerts a protective effect against high glucose/hypoxia‑induced injury in aortic endothelial cells through its antioxidative action, which may involve the inhibition of the PKC pathway and regulation of HIF-1α.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang SL, Liu DS, Liang ES, Gao YH, Cui Y, Liu YZ and Gao W: Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress. Exp Ther Med 10: 1394-1400, 2015.
APA
Wang, S., Liu, D., Liang, E., Gao, Y., Cui, Y., Liu, Y., & Gao, W. (2015). Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress. Experimental and Therapeutic Medicine, 10, 1394-1400. https://doi.org/10.3892/etm.2015.2708
MLA
Wang, S., Liu, D., Liang, E., Gao, Y., Cui, Y., Liu, Y., Gao, W."Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress". Experimental and Therapeutic Medicine 10.4 (2015): 1394-1400.
Chicago
Wang, S., Liu, D., Liang, E., Gao, Y., Cui, Y., Liu, Y., Gao, W."Protective effect of allicin on high glucose/hypoxia-induced aortic endothelial cells via reduction of oxidative stress". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1394-1400. https://doi.org/10.3892/etm.2015.2708