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Inhibition of duck hepatitis B virus replication by mimic peptides in vitro

  • Authors:
    • Hongyu Jia
    • Changhong Liu
    • Ying Yang
    • Haihong Zhu
    • Feng Chen
    • Jihong Liu
    • Linfu Zhou

  • View Affiliations

  • Published online on: September 21, 2015     https://doi.org/10.3892/etm.2015.2757
  • Pages: 1697-1703

  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the inhibitory effect of specific mimic peptides targeting duck hepatitis B virus polymerase (DHBVP) on duck hepatitis B virus (DHBV) replication in primary duck hepatocytes. Phage display technology (PDT) was used to screen for mimic peptides specifically targeting DHBVP and the associated coding sequences were determined using DNA sequencing. The selected mimic peptides were then used to treat primary duck hepatocytes infected with DHBV in vitro. Infected hepatocytes expressing the mimic peptides intracellularly were also prepared. The cells were divided into mimic peptide groups (EXP groups), an entecavir‑treated group (positive control) and a negative control group. The medium was changed every 48 h. Following a 10‑day incubation, the cell supernatants were collected. DHBV‑DNA in the cellular nucleus, cytoplasm and culture supernatant was analyzed by quantitative polymerase chain reaction (qPCR). Eight mimic peptides were selected following three PDT screening rounds for investigation in the DHBV‑infected primary duck hepatocytes. The qPCR results showed that following direct treatment with mimic peptide 2 or 7, intracellular expression of mimic peptide 2 or 7, or treatment with entecavir, the DHBV‑DNA levels in the culture supernatant and cytoplasm of duck hepatocytes were significantly lower than those in the negative control (P<0.05). The cytoplasmic DHBV‑DNA content of the cells treated with mimic peptide 7 was lower than that in the other groups (P<0.05). In addition, the DHBV‑DNA content of the nuclear fractions following the intracellular expression of mimic peptide 7 was significantly lower than that in the other groups (P<0.05). Mimic peptides specifically targeting DHBVP, administered directly or expressed intracellularly, can significantly inhibit DHBV replication in vitro.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jia H, Liu C, Yang Y, Zhu H, Chen F, Liu J and Zhou L: Inhibition of duck hepatitis B virus replication by mimic peptides in vitro. Exp Ther Med 10: 1697-1703, 2015.
APA
Jia, H., Liu, C., Yang, Y., Zhu, H., Chen, F., Liu, J., & Zhou, L. (2015). Inhibition of duck hepatitis B virus replication by mimic peptides in vitro. Experimental and Therapeutic Medicine, 10, 1697-1703. https://doi.org/10.3892/etm.2015.2757
MLA
Jia, H., Liu, C., Yang, Y., Zhu, H., Chen, F., Liu, J., Zhou, L."Inhibition of duck hepatitis B virus replication by mimic peptides in vitro". Experimental and Therapeutic Medicine 10.5 (2015): 1697-1703.
Chicago
Jia, H., Liu, C., Yang, Y., Zhu, H., Chen, F., Liu, J., Zhou, L."Inhibition of duck hepatitis B virus replication by mimic peptides in vitro". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1697-1703. https://doi.org/10.3892/etm.2015.2757