Enhanced expression of glucose‑regulated protein 78 correlates with malondialdehyde levels during the formation of liver cirrhosis in rats

Zhang,Yun; Zhang,Huiying; Zhao,Zhongfu; Lv,Minli; Jia,Jiantao; Zhang,Lili; Tian,Xiaoxia; Chen,Yunxia; Li,Baohong; Liu,Mingshe; Han,Dewu; Ji,Cheng

doi:10.3892/etm.2015.2783

Enhanced expression of glucose‑regulated protein 78 correlates with malondialdehyde levels during the formation of liver cirrhosis in rats

Authors:
- Yun Zhang
- Huiying Zhang
- Zhongfu Zhao
- Minli Lv
- Jiantao Jia
- Lili Zhang
- Xiaoxia Tian
- Yunxia Chen
- Baohong Li
- Mingshe Liu
- Dewu Han
- Cheng Ji
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Affiliations: Institute of Hepatology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Pathophysiology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, ICU of The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Microbiology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Institute of Hepatology, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, USC Research Center for Liver Disease, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA
Published online on: October 1, 2015 https://doi.org/10.3892/etm.2015.2783
Pages: 2119-2125
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to explore the role of glucose‑regulated protein 78 (GRP78) in the development of liver cirrhosis promoted by intestinal endotoxemia in rats. Fifty‑one male Wistar rats were randomly divided into the liver cirrhosis 4‑week, 6‑week and 8‑week groups and the normal control group at each time point. Liver cirrhosis was induced by employing multiple pathogenic factors in the rats. Blood and liver tissues were collected. The levels of alanine aminotransferase (ALT), homocysteine, endotoxin and tumor necrosis factor‑α (TNF‑α) in the plasma, and TNF‑α, malondialdehyde (MDA) and procollagen type III peptide (PIIIP) in the liver tissues were determined. The mRNA and protein expression levels of GRP78 in the liver were detected using reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry. Morphological changes were observed through hematoxylin and eosin and van Gieson staining of the liver. Liver cirrhosis caused marked histopathological changes to the livers of the rats. Following significant increases in the levels of ALT, homocysteine, endotoxin and TNF‑α in the plasma, and TNF‑α, MDA and PIIIP in the liver tissues of all experimental groups with the progression of liver cirrhosis, the mRNA and protein expression levels of GRP78 also gradually increased. In addition, correlation analysis indicated that the enhanced expression of GRP78 correlated with the MDA levels of the rats during the formation of liver cirrhosis.

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