Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase in Enterobacter cloacae

Liu,Jun; Li,Guo‑Ming; Lin,Li‑Yao; Wu,Xia‑Lei; Huang,Shao‑Long; Zhou,Yong; Zhao,Zu‑Guo

doi:10.3892/etm.2015.2851

Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase in Enterobacter cloacae

Authors:
- Jun Liu
- Guo‑Ming Li
- Li‑Yao Lin
- Xia‑Lei Wu
- Shao‑Long Huang
- Yong Zhou
- Zu‑Guo Zhao
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Affiliations: Laboratory of Pathogenic Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China, Department of Cardiothoracic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. China, Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. China, Department of Experimental Medicine, Taiping People's Hospital of Dongguan, Dongguan, Guangdong 523095, P.R. China
Published online on: November 10, 2015 https://doi.org/10.3892/etm.2015.2851
Pages: 269-276

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Abstract

The association between antibiotic resistance and SHV-12 extended-spectrum β-lactamase (ESBL) in Enterobacter cloacae remains unknown. The aim of the present study was to investigate the prevalence of both chromosome- and plasmid-borne SHV-12 ESBL genes in Enterobacter cloacae. Transmission of the SHV‑12 ESBL gene was explored, and the risk factors for antibiotic resistance in E. cloacae were analyzed. Polymerase chain reaction (PCR) results showed that 58 out of the 100 isolates carried the SHV‑12 ESBL gene: 34.48% of them occurred in the chromosome, 48.28% were plasmid‑borne and 17.24% appeared in both. Enterobacterial repetitive intergenic consensus‑PCR tests detected 82 chromosomal genotypes. Conjugation assays showed that 70.00% of plasmid‑borne SHV‑12 ESBL genes were successfully transconjugated into E. coli C600 and that the antibiotic resistance phenotype of E. cloacae was partially (84%) or completely (10%) transferred. A significantly higher SHV‑12 ESBL detection rate was found in patients with underlying conditions and/or complications compared with those without (P<0.05). The detection of SHV‑12 ESBL‑producing E. cloacae from vertical transmission varied significantly across clinical departments and age groups (P<0.05), with the highest rates in the intensive care unit and the group of patients aged ≥60 years. The present results indicate that the location and transmission efficiency of SHV-12 ESBL are closely correlated with the antibiotic resistance of E. cloacae.

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